Nestin Delineates Pancreatic Cancer Stem Cells in Metastatic Foci of NODShi-scid IL2Rγnull (NOG) Mice》.pdfVIP

The American Journal of Pathology, Vol. 184, No. 3, March 2014 GASTROINTESTINAL, HEPATOBILIARY, AND PANCREATIC PATHOLOGY Nestin Delineates Pancreatic Cancer Stem Cells in Metastatic Foci of NOD/Shi-scid IL2Rgnull (NOG) Mice Yoko Matsuda, * Hisashi Yoshimura,* Junji Ueda, *y Zenya Naito,* Murray Korc,z and Toshiyuki Ishiwata* From the Departments of Pathology and Integrative Oncological Pathology,* and the Department of Surgery for Organ and Biological Regulation,y Graduate School of Medicine, Nippon Medical School, Tokyo, Japan; and the Departments of Medicine and Biochemistry and Molecular Biology,z Indiana University School of Medicine and the Melvin and Bren Simon Cancer Center, Indianapolis, Indiana Accepted for publication November 18, 2013. Pancreatic ductal adenocarcinoma (PDAC) is associated with a high incidence of hepatic metastases, as well as occasional pulmonary metastases. To delineate the potential role of cancer stem cells (CSCs) in PDAC Address correspondence to metastasis, human PDAC cells were injected into the spleen of mice. The characteristics and expression of Toshiyuki Ishiwata, M.D., Ph.D., Director of Molecular markers associated with CSC and epithelialemesenchymal transition (EMT) of metastatic cells that Pathology Group, Departments developed in the liver and lung were then compared with parental cells. The metastatic cells were polyg- of Pathology and Integrative onal, and larger than parental cells. Metastatic cells also exhibited decreased proliferation and increased Oncological Pathology, Nippon adhesion to extracellular matr