miR-483–5p and miR-486–5p are down-regulated in cumulus cells of metaphase II oocytes from women with polycystic ovary syndrome》.pdfVIP

Reproductive BioMedicine Online (2015) 31, 565–572 ARTICLE miR-483–5p and miR-486–5p are down-regulated in cumulus cells of metaphase II oocytes from women with polycystic ovary syndrome Lin Shi a,1, Shan Liu b,1, Wanqiu Zhao b, Juanzi Shi b,* a Department of Immunology and Microbiology, Xi’an Jiaotong University Health Science Center, Xi’an 710061, China; b Assisted Reproduction Center, Maternal and Child Health Care Hospital of Shaanxi Province, Xi’an 710003, China * Corresponding author. E-mail address: shijuanzi123@126.com (J Shi). 1 These authors have contributed equally to this paper. Dr Juanzi Shi has been the Director of the Assisted Reproduction Center, Maternal and Child Health Care Hos- pital of Shaanxi Province since 2006. She received her PhD in Pathology in 2003 from the Fourth Military Medical University. She is Professor of Physicians in the IVF unit and has 5 years’ experience in IVF laboratory work. Her primary area of research is embryology and assisted reproductive technology. She has published several ar- ticles and book chapters about the vitrification of human embryos. Abstract The aim of this study was to compare the expression of microRNAs (miRNAs) in cumulus cells from polycystic ovary syn- drome (PCOS) and non-PCOS women. In the present study, miRNA expression profiles of the cumulus cell samples were determined by miRNA microarrays. Quantification of selected miRNAs and predicted target genes was performed using quantitative real-time PCR (qRT-PCR). The results showed that miR-483–5p and miR-486–5p are significantly decreased in cumulus cells of PCOS patients PCOS (fold change 2, false discovery rate 0.001). qRT-PCR found that four predicted genes, SOCS3, SRF, PTE