Lipid hydroperoxide-induced and hemoglobin-enhanced oxidative damage to colon cancer cells》.pdfVIP

Free Radical Biology Medicine 51 (2011) 503–515 Contents lists available at ScienceDirect Free Radical Biology Medicine journal homepage: www. elsevier.c om/l oc ate/ freeradbiomed Original Contribution Lipid hydroperoxide-induced and hemoglobin-enhanced oxidative damage to colon cancer cells José Pedro F. Angeli, Camila Carrião M. Garcia, Fernanda Sena, Florencio P. Freitas, Sayuri Miyamoto, Marisa Helena G. Medeiros, Paolo Di Mascio ⁎ Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, CEP 05508–000 São Paulo, SP, Brazil a r t i c l e i n f o a b s t r a c t Article history: Epidemiological studies have indicated that Western diets are related to an increase in a series of Received 16 October 2010 malignancies. Among the compounds that are credited for this toxic effect are heme and lipid peroxides. We Revised 5 April 2011 evaluated the effects of hemoglobin (Hb) and linoleic acid hydroperoxides (LAOOH) on a series of Accepted 8 April 2011 toxicological endpoints, such as cytotoxicity, redox status, lipid peroxidation, and DNA damage. We Available online 14 April 2011 demonstrated that the preincubation of SW480 cells with Hb and its subsequent exposure to LAOOH (Hb + LAOOH) led to an increase in cell death, DCFH oxidation, malonaldehyde formation, and DNA fragmentation Keywords: Lipid hydroperoxid