Lipid packing determines protein–membrane interactions Challenges for apolipoprotein A-I and high density lipoproteins》.pdfVIP

Biochimica et Biophysica Acta 1798 (2010) 1399–1408 Contents lists available at ScienceDirect Biochimica et Biophysica Acta journa l homepage: /locate/bbamem Review Lipid packing determines protein–membrane interactions: Challenges for apolipoprotein A-I and high density lipoproteins Susana A. Sánchez a,⁎, M. Alejandra Tricerri b, Giulia Ossato a, Enrico Gratton a a Laboratory for Fluorescence Dynamics (LFD), University of California at Irvine, Biomedical Engineering Department, 3120 Natural Sciences 2, Irvine, CA 92697-2715, USA b Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP), Universidad Nacional de La Plata, La Plata, Argentina a r t i c l e i n f o a b s t r a c t Article history: Protein and protein–lipid interactions, with and within specific areas in the cell membrane, are critical in Received 22 August 2009 order to modulate the cell signaling events required to maintain cell functions and viability. Biological Received in revised form 18 March 2010 bilayers are complex, dynamic platforms, and thus in vivo observations usually need to be preceded by Accepted 19 March 2010 studies on model systems that simplify and discriminate the different factors involved in lipid–protein Available online 27 March 2010 interactions. Fluorescence microscopy studies using giant unilamellar vesicles (GUVs) as membrane model