Lipidation of apolipoprotein A-I by ATP-binding cassette transporter (ABC) A1 generates an interaction partner for ABCG1 but not for scavenger receptor BI》.pdfVIP

Biochimica et Biophysica Acta 1781 (2008) 306–313 Contents lists available at ScienceDirect Biochimica et Biophysica Acta journa l homepage: /locate/bbalip Lipidation of apolipoprotein A-I by ATP-binding cassette transporter (ABC) A1 generates an interaction partner for ABCG1 but not for scavenger receptor BI Iris Lorenzi, Arnold von Eckardstein, Silvija Radosavljevic, Lucia Rohrer ⁎ Institute of Clinical Chemistry and Center for Integrative Human Biology, University of Zurich, University Hospital Zurich, Rämistrasse 100, 8091 Zurich, Switzerland A R T I C L E I N F O A B S T R A C T Article history: The ATP-binding cassette transporters ABCA1 and ABCG1 as well as scavenger receptor BI (SR-BI) mediate the Received 28 February 2008 efflux of lipids from macrophages to apolipoprotein A-I (apoA-I) and high density lipoproteins (HDL). We Received in revised form 2 April 2008 used RNA interference in RAW264.7 macrophages to study the interactions of ABCA1, ABCG1, and SR-BI with Accepted 17 April 2008 lipid-free apoA-I, native and reconstituted HDL with apoA-I:phosphatidylcholine ratios of either 1:40 Available online 26 April 2008 (rHDL1:40) or 1:100 (rHDL1:100). Knock-down of ABCA1 inhibits the cellular binding at 4 °C of lipid-free apoA-I Keywords: but not of HDL whereas suppression of ABCG1 or SR-BI reduces the binding of HDL but not lipid-free apoA-I. ABC1