Modeling of cancer metastasis and drug resistance via biomimetic nano-cilia and microfluidics》.pdfVIP

Biomaterials 35 (2014) 1562e1571 Contents lists available at ScienceDirect Biomaterials journal homepage: www.elsevier .com/locate/biomaterials Modeling of cancer metastasis and drug resistance via biomimetic nano-cilia and microfluidics Ching-Te Kuo a, Chi-Ling Chiang b, Chi-Hao Chang c, Hao-Kai Liu a, Guan-Syuan Huang a, Ruby Yun-Ju Huang d, e, Hsinyu Lee c, *, Chiun-Sheng Huang f, *, Andrew M. Wo a, * a Institute of Applied Mechanics, National Taiwan University, Taipei, Taiwan, ROC b School of Biomedical Science, Ohio State University, Columbus, USA c Department of Life Science, National Taiwan University, Taipei, Taiwan, ROC d Department of Obstetrics Gynaecology, National University Hospital, Singapore e Cancer Science Institute of Singapore, National University of Singapore, Singapore f Department of Surgery, National Taiwan University Hospital, Taipei, Taiwan, ROC a r t i c l e i n f o a b s t r a c t Article history: Three-dimensional (3D) tissue culture platforms that are capable of mimicking in vivo microenviron- Received 7 October 2013 ments to replicate physiological conditions are vital tools in a wide range of cellular and clinical studies. Accepted 2 November 2013 Here, learning from the nature of cilia in lungs e clearing mucus and pathogens from the airway e we Available online 22 November 2013 develop a 3D culture approach via flexible and kinetic copolymer-based chains (nano-cilia) for dimin- ishing cell-to-substrate adh