miR-21 as an Independent Biochemical Recurrence Predictor and Potential Therapeutic Target for Prostate Cancer》.pdfVIP

miR-21 as an Independent Biochemical Recurrence Predictor and Potential Therapeutic Target for Prostate Cancer Tao Li, Run-Sheng Li, Yu-Hua Li, Shang Zhong, Yu-Ying Chen, Cun-Ming Zhang, Ming-Ming Hu and Zhou-Jun Shen* From the Department of Urology (TL, SZ, CMZ, MMH, ZJS), Rui Jin Hospital, Shanghai Jiao Tong University, Shanghai Institute of Planned Parenthood Research (RSL, YHL) and Department of Biochemistry and Cell Biology (YYC), Key Laboratory of the Education Ministry for Cell Differentiation and Apoptosis, Institute of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China Abbreviations Purpose: Abnormal miRNA expression is associated with prostate cancer pro- and Acronyms gression. However, the relationship between miRNA and biochemical recurrence BCR biochemical recurrence after radical prostatectomy is not well established. Thus, we evaluated the miRNA miR-21 as a biomarker to predict the risk of biochemical failure, and as ISH in situ hybridization a potential drug target for prostate cancer therapy. LNA locked nucleic acid Materials and Methods: miR-21 levels were assayed using locked nucleic acid in MARCKS myristoylated situ hybridization coupled with tissue microarray techniques in 169 radical alanine-rich protein kinase C prostatectomy tissue samples. The Cox proportional hazard model was used to substrate analyze miR-21 expression as an independent predictor of biochemical recur- miRNA microRNA rence. The association of miR-21 with recurrence was estimated using the Kap- PBS pho