Metagenomic insights into the effects of volatile fatty acids on microbial community structures and functional genes in organotrophic anammox process》.pdfVIP

Bioresource Technology 196 (2015) 621–633 Contents lists available at ScienceDirect Bioresource Technology journal homepage: www.elsevi /locate/biortech Metagenomic insights into the effects of volatile fatty acids on microbial community structures and functional genes in organotrophic anammox process Duntao Shu a, Yanling He b,⇑, Hong Yue c, Liang Zhu d, Qingyi Wang e a Center for Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of the Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University, Shaanxi 710049, China b School of Human Settlements Civil Engineering, Xi’an Jiaotong University, Shaanxi 710049, China c State Key Laboratory of Crop Stress Biology in Arid Areas, College of Agronomy and Yangling Branch of China Wheat Improvement Center, Northwest AF University, Yangling, Shaanxi 712100, China d Institute of Environmental Biotechnology, Suzhou University of Science and Technology, Suzhou 215009, China e School of Chemical Engineering Technology, Xi’an Jiaotong University, Shaanxi 710049, China h i g h l i g h t s Lower VFAs levels had no significantly effects on anammox activity. ‘‘Ca. Brocadia sinica” has the capacity to oxidize the VFAs. Microbial communities were investigated by Miseq pyrosequencing and qPCR. Coupling of anammox, denitrification and DNRA were confirmed in this study. Different VFAs had highly impacts on the microbial communities. a r t i c l e i n f o a b s t r a c t Article history: To explore the metabolic versatility of ‘‘Candidatus Brocadia sinica” in the presence of VFAs, the impacts Received 14 June 2015 of VFAs on anammox activity and nitroge