Methotrexate chemotherapy triggers touch-evoked pain and increased CGRP-positive sensory fibres in the tibial periosteum of young rats》.pdfVIP

Bone 73 (2015) 24–31 Contents lists available at ScienceDirect Bone journal homepage: /locate/bone Original Full Length Article Methotrexate chemotherapy triggers touch-evoked pain and increased CGRP-positive sensory fibres in the tibial periosteum of young rats Fiona H. Zhou, Yingnan Yu, Xin-Fu Zhou, Cory J. Xian ⁎ Sansom Institute for Health Research, School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, SA 5001, Australia a r t i c l e i n f o a b s t r a c t Article history: Although bone pain caused by cancer chemotherapy is a well-recognized and significant problem, with approx- Received 7 June 2014 imately 1 in 10 childhood cancer patients being reported to experience isolated bone pain along with other Revised 19 November 2014 skeletal complications, the underlying mechanisms are poorly understood and there is no specific treatment. Accepted 20 November 2014 In this study, effects of methotrexate (MTX) treatment on pain in the hind legs and the extent of sensory inner- Available online 3 December 2014 vation of the tibial bone were examined through a 20-day time course in young rats after 5 daily 0.75 mg/kg MTX Edited by: David Burr injections. MTX treatment increased von-Frey filament stimulation-induced mechanical allodynia and palpation nocifensive score in the tibia. MTX-treated rats showed trends