3,4,5-三羟基苯甲酸印迹聚合物分子识别特性.pdfVIP

第 33 卷第 2 期 2011 年 3 月 Vol. 33 No. 2 Journal of Tangshan Teachers College Mar. 2011 化学研究 3,4,5-三羟基苯甲酸印迹聚合物的分子识别特性 张铁莉 （唐山师范学院化学系，河北 唐山 063000 ） 摘 要：采用非共价印迹技术，以 3,4,5-三羟基苯甲酸（3,4,5-THBA ）为印迹分子，丙烯酰胺为功能单体， 乙二醇二甲基丙烯酸酯为交联剂，乙腈为致孔剂，60 ℃热引发制备了印迹聚合物 P(3,4,5-THBA) ，用高效液相色 谱法和 Scatchard 作图评价了其选择性结合特性。结果表明，P(3,4,5-THBA)对 3,4,5-THBA 具有选择性结合能力， 氢键结合在其分子识别过程中起主要作用，其中存在两类结合位点，高亲和力结合位点的离解常数 KD1=2.4 × -4 -3 10 mol/L ，最大结合位点数Qmax1=30.6μmol/g ；低亲合力结合位点的离解常数 KD2=2.3 ×10 mol/L ，最大结合位点 数 Qmax2=91.1μmol/g 。 关键词：分子印迹聚合物；分子识别；3,4,5-三羟基苯甲酸；Scatchard 分析 TQ050.4+25 A 1009-9115(2011)02-0001-03 中图分类号： 文献标识码： 文章编号： Molecular Recognition Performances of 3,4,5-Trihydroxybenzoic Acid-Imprinted Polymer ZHANG Tie-li (Department of Chemistry, Tangshan Teachers College, Tangshan 063000, China) Abstract: 3,4,5-Trihydroxybenzoic acid (3,4,5-THBA)-imprinted polymer P(3,4,5-THBA) was synthesized thermally initiated at 60℃ by non-covalent molecular imprinting technique using 3,4,5-THBA as template molecule, acrylamide as functional monomer, ethyleneglycol dimethacrylate as cross-linker, and acetonitrile as porogenic solvent. The specific rebinding characteristics of P(3,4,5-THBA) for 3,4,5-THBA were investigated via high-performance liquid chromatography and Scatchard analysis. It was revealed that P(3,4,5-THBA) showed high specific affinity to 3,4,5-THBA and hydrogen bonding played a major role and that two kinds of sites existed on the polymer matrix with their dissociation constants being est