液相芯片同步分型检测猪传染性胸膜肺炎抗体方法的建立和应用.pdfVIP

上海市畜牧兽医学会2011 年学术年会论文集  液相芯片同步分型检测猪传染性胸膜肺炎 抗体方法的建立和应用 王 艳，李树清，杜 军，陈志飞，王巧全 （上海出入境检验检疫局，上海 200135） 摘 要：目的 基于液相芯片技术建立一种对猪传染性胸膜肺炎抗体同步分型检测的新方法。 方法 制备抗体 交联微球及生物素标记抗体，用双抗体夹心法测定临床血清样品，并对其特异性和敏感性进行检测。结果 检 测结果显示，该方法可以同时检测猪血清中 7 种 App 抗体，具有较好的特异性；检测灵敏度也普遍高于现有 商品化 App ELISA 检测试剂盒。结论 初步建立了同步分型检测 App 的液相芯片技术，为进一步同步检测 15 个血清型奠定了基础，同时也可以满足进出口种猪检疫，快速、准确的要求。 关键词：猪传染性胸膜肺炎；液相芯片；同步检测 PRELIMINARY DEVELOPMENT OF LIQUICHIP DETECTION TECHNIQUE FOR SIMULTANEOUS DETECTION OF ANTIBODIES OF ACTIONBACILLUS PLEUROPNEUMONIAE Wang Yan, Li Shu-qing, Du Jun, Chen Zhi-fei, Wang Qiao-quan (Shanghai Entry-Exit Inspection and Quarantine, Shanghai, 200135) Abstract: The aim is to develop Liquichip detection technique to simultaneously detect antibodies of different serotypes of Actinobacillus pleuropneumoniae. Methods: Antibody-conjugated microspheres and biotinylated detection antibodies were prepared. A sandwich immunoassay was established to measure the levels of App. We also conduct the sensitive and specific detection of this assay. Results: The results showed that this assay method could simultaneous detection of 7 serotypes of App. The results also displayed reasonable specificity and sensitivity when being used for this assay. And the results of sensitive detection also showed that this assay was more sensitive than commercial ELISA Kit. Conclusion: The Liquichip detection technique to detect App was establised preliminary, which provided a foundation for further research on rapidly high throughout detection for 15 different serotypes of App and can be used in Entry-Exit quarantine and inspection. Key words: Actinobacillus pleuropneumoniae ; Liquichip detection technique; simultaneous   1