苯酚_硫酸法测定无花果中多糖含量的研究.pdfVIP

食品科技 食品安全与检测 FOOD SCIENCE AND TECHNOLOGY 2009 年第34 卷第 10 期 苯酚－硫酸法测定无花果中 多糖含量的研究 1,2 1 2 1 2 郁 玮 ， 杨小明 ， 刘伟民 ， 刘 方 ， 马海乐 (1.江苏大学化学化工学院，镇江212013 ； 2.江苏大学食品与生物技术学院，镇江212013) 摘要： 分别以单一葡萄糖及按无花果多糖中单糖组成比例配制的混合单糖为标准糖样，采用苯 酚－硫酸法对无花果多糖的含量进行测定。结果表明，以单一葡萄糖为标准品测得含量偏低，而 以混合糖为标准品不仅重现性高、稳定性好，而且回收率实验理想，可认为是测定无花果多糖含 量较为简便的方法。 关键词： 无花果多糖；苯酚－硫酸法；含量测定 中图分类号： TS 255.1 文献标志码： A 文章编号： 1005-9989(2009)10-0256-03 Assay study on content of polysaccharides in ficus carica by phenol-sulfuric acid method 1,2 1 2 1 2 YU Wei , YANG Xiao-ming , LIU Wei-min , LIU Fang , MA Hai-le (1.School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013; 2.School of Food Biological Engineering, Jiangsu University, Zhenjiang 212013) Abstract: The content of polysaccharides in Ficus carica was determined by the method of phenol -sulfuric acid, according the proportion of sugars in the Ficus carica polysaccharide to obtain the mixture of Ficus carica polysaccharide compositions, then taking the single glucose and the mixture sugar as standard solution respectively. The results showed the content of polysaccharides in Ficus carica was lower using single glucose as standard solution, but the method was steadier and had better reproducibility and recovery rate using the reference of sugar mixture, so this method can be used to determine the Ficus carica polysaccharides simply and accurately.