江浙莼菜遗传多样性和遗传结构的ISSR 分析[J].pdfVIP

J. Lake Sci.(湖泊科学), 2008, 20(5): 662-668 . E-mail: jlakes@ ©2008 by Journal of Lake Sciences * 江浙莼菜遗传多样性和遗传结构的 ISSR 分析 张光富 1,2, 高邦权 1 (1: 南京师范大学生命科学学院, 南京 210046) (2: 中国科学院南京地质古生物研究所, 现代古生物学和地层学国家重点实验室, 南京 210008) 摘 要: 利用ISSR 标记对分布于江苏和浙江的濒危水生植物莼菜(Brasenia schreberi J. F. Gmelin) 的遗传多样性和遗传结构进 行研究. 从77 个引物中筛选出 12 个引物用于研究, 共扩增出 101 条扩增带, 其中多态性条带 16 条, 占15.84%, 平均每个引 物扩增的条带数为8.4 条. 结果表明: 莼菜的遗传多样性水平较低(种水平的遗传多样性Ht 为0.0395, Shannon’s 信息指数Hs 为0.0630; 种群水平的遗传多样性Ht 、Hs 分别为0.0391、0.0335). 大部分遗传变异存在于种群内(85.79%), 种群间的基因流 为3.0199. 营养繁殖、生境丧失与片断化以及人工引种等人为干扰对莼菜的遗传多样性和遗传结构产生重要影响. 关键词: 莼菜; 遗传多样性; 遗传结构; ISSR Analysis on genetic diversity and genetic structure of Brasenia schreberi in Jiangsu and Zhejiang Provinces revealed by ISSR Markers ZHANG Guangfu1,2 GAO Bangquan1 (1: School of Life Sciences, Nanjing Normal University , Nanjing 210046, P.R.China) (2: State Key Laboratory of Palaeobiology and Stratigraphy, Nanjing Institute of Geology and Palaeontology, Chinese Academy of Sciences, Nanjing 210008, P.R.China) Abstract : In order to investigate the level of genetic diversity of the endangered species Brasenia schreberi , three populations from Jiangsu and Zhejiang Provinces were sampled and analyzed using inter-simple sequence repeats (ISSR) markers. A total of fifty-four individuals were sampled. Twelve filtrated from 77 ISSR primers gave rise to 101 discernible DAN fragments of which 16 were polymorphic (15.84%). The average number of DNA bands amplified by each primer was 8.4. The Nei’s genetic and Shannon’s information indexes were 0.0395 and 0.0630 respectively at species level, 0.0391 and 0.0335 respectively at population level. Based on Nei’s Gst value an