巨磁阻效应微流控传感器在肿瘤诊断中应用的研究.docVIP

巨磁阻效应的微流控传感器在肿瘤诊断中的应用研究 崔大祥，郅晓，杨浩，张昕，张雪青，王侃 上海交通大学微纳米科学技术研究院，微纳制造技术国家重点实验室，薄膜与微细技术教育部重点实验室，上海市东川路800号，邮编：200240，E-mail: dxcui@sjtu.edu.cn 这里我们报道我们课题组近3年来，在磁性纳米团簇的制备、巨磁阻效应传感器的研制以及结合PCR技术与微流控芯片技术，成功地研制出巨磁阻效应的微流控传感器件，并进行了传感器件在捕获血液样本中癌细胞、肿瘤相关病毒的基因分型检测中的应用研究。结果表明：研制的传感器能够快速捕获血液中的胃癌细胞，并能定量，能够快速区分人乳头瘤病毒16与18基因型,也能够用于我国肝炎病毒的快速基因分型。结论：基于磁性纳米粒子标记与巨磁阻效应的微流控传感器在循环血液中肿瘤细胞的检测、肿瘤相关的病毒基因分型诊断中具有广阔的应用前景。 关键词： 磁性纳米粒子；巨磁阻效应；传感器；微流控系统；肿瘤；病毒；基因分型 参考文献： Zhi X, Liu Q, Zhang X, Zhang Y, Feng J, Cui DX(. Quick genotyping detection of HBV by giant magnetoresistive biochip combined with PCR and line probe assay. Lab on a chip 2012:12(4):741-745. Chen, L., Bao, C.-C., Yang, H., Li, D., Lei, C., Wang, T., Hu, H.-Y., He, M., Zhou, Y., and Cui, D.-X. (2011) A prototype of giant magnetoimpedance-based biosensing system for targeted detection of gastric cancer cells, Biosensors and Bioelectronics 26, 3246-3253. Yang, H., Chen, L., Lei, C., Zhang, J., Li, D., Zhou, Z.-M., Bao, C.-C., Hu, H.-Y., Chen, X., Cui, F., Zhang, S.-X., Zhou, Y., and Cui, D.-X. (2010) Giant magnetoimpedance-based microchannel system for quick and parallel genotyping of human papilloma virus type 16/18, Applied Physics Letters 97, 043702-043703. Hu, H., Yang, H., Li, D., Wang, K., Ruan, J., Zhang, X., Chen, J., Bao, C., Ji, J., Shi, D., and Cui, D. (2010) The potential of magnetic nanocluster and dual-functional protein-based strategy for noninvasive detection of HBV surface antibodies, Analyst 136, 679-683. Hu, H., Yang, H., Huang, P., Cui, D., Peng, Y., Zhang, J., Lu, F., Lian, J., and Shi, D. (2010) Unique role of ionic liquid in microwave-assisted synthesis of monodisperse magnetite nanoparticles, Chemical Communications 46, 3866-3868. Gao, G., Huang, P., Zhang, Y., Wang, K., Qin, W., and Cui, D. (2011) Gram scale synthesis of superparamagnetic Fe(3)O(4) nanoparticles and fluid via a facile solvothermal route, Crystengcomm 13, 1782-1785. Giant Magnetic Resistance Effects- based Microfluidic Biosensors applied to Tum