高效液相色谱法检测甘蓝中残留2,5-二苯乙炔基噻吩的方法研究.docVIP

 n 高效液相色谱法检测甘蓝中残留 2,5-二苯乙 炔基噻吩的方法研究# 陈小军，范淑琴，王萌，杨益众** 5 10 15 20 25 （扬州大学园艺与植物保护学院，江苏 扬州 225009） 摘要：2,5-二苯乙炔基噻吩是一种优异的光敏剂，对害虫具有良好的光敏毒杀作用，可以用 于防治蔬菜害虫。甘蓝中的 2,5-二苯乙炔基噻吩以乙腈为提取溶剂，采用超声波辅助提取、 经净化，以高效液相色谱（HPLC）分析甘蓝中残留的 2,5-二苯乙炔基噻吩。[结果]研究表 明甘蓝中 2,5-二苯乙炔基噻吩的添加回收率为 83.04%～89.71%，变异系数为 1.56%~3.43%， 最低检测质量分数 0.01 μg/g。本方法检测 2,5-二苯乙炔基噻吩，具有检测快速，准确等 优点，且重现性、稳定性、分离效果均能满足分析要求，是一种较为理想的分析方法。 关键词：农药学；2,5-二苯乙炔基噻吩；高效液相色谱；甘蓝；分析 中图分类号：X592 Determination of 2, 5-Diphenylethynylthiophene in the Cabbage by High Performance Liquid Chromatography CHEN Xiaojun, FAN Shuqin, WANG Meng, YANG Yizhong (School of Horticulture and Plant Protection,Yangzhou University, JiangSu YangZhou 225009) Abstract: 2,5-Diphenylethynylthiophene is a good photosensitizer and high phototoxicity to a variety of insects and which could be used to control vegetable pests. [Methods]The acetonitrile was used as extracted solvent and 2,5-diphenylethynylthiophene in the cabbage was extracted with the ultrasonic extracted method, purified and content of 2,5-diphenylethynylthiophene in the cabbage was analyzed by high performance liquid chromatography(HPLC). [Results]The results indicated that average recovery of 2,5-diphenylethynylthiophene in the cabbage was 83.04%~89.71%, coefficient variance was 1.56%~3.43%, respectively and the lowest detection concentration was 0.01 μg/g.Extraction method of 2,5-diphenylethynylthiophene from the cabbahe and its analysis method accorded to pesticide residues criterion and which was simple, rapid, accurate, reproducibility, stability, separation effect and convenient to operate.In this paper. Keywords: pesticide; 2,5-diphenylethynylthiophene; HPLC; cabbage; determination 30 0 引言 光活化农药是近年来研究的热点，进入 90 年代后，我国也开始了对光活化杀虫剂的研 究。1993 年，徐汉虹等首次报道了猪毛蒿（Artemisia scoparia）精油中的茵陈二炔对斜纹夜 蛾（Spodoptera litura）的光活化毒杀活性[1]，代表国内研究光活化杀虫剂的开端，此后国内 35 40 各研究单位开始了对光活化杀虫剂的系统研究[2-11]。虽说三联噻吩（?-T）对多种昆虫具有 良好的光活化毒杀活性，但自从?-T 光活化活性被发现已经有 30 多年了，一直没能大规模 田间应用，主要原因是其在环境中半衰期太短，合成难度较大，成本高、工业化生产面临很 多问题。为了克服?-T 等光活化农药应用于田间