基于正交试验的野木瓜中反丁烯二酸提取工艺的优化（职称论文资料）.docVIP

基于正交试验的野木瓜中反丁烯二酸提取工艺的优化（职称论文资料） 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：基于正交试验的野木瓜中反丁烯二酸提取工艺的优化 1 1 仪器与试药 3 2 方法与结果 3 3 讨论 7 文2：肿节风药材中反丁烯二酸提取工艺的研究 8 1 器材 9 2 方法 10 3 结果 11 参考文摘引言： 12 原创性声明（模板） 13 文章致谢（模板） 14 正文 基于正交试验的野木瓜中反丁烯二酸提取工艺的优化（职称论文资料） 文1：基于正交试验的野木瓜中反丁烯二酸提取工艺的优化 Optimization of the extraction technics for fumaric acids from Starntonia chineis DC. based on orthogonal test HU Shuping， LIU Tongxiang， LIU Shijie Ititute of Chinese Minority Traditional Medical， Minzu Univeity of China， Beijing 100081， China [Abstract] Objective: To explore the best extraction conditio of fumaric acid in Starntonia chineis DC. by using HPLC and orthogonal test L9(34). Methods: Orthogonal test was applied to probe the extraction process on dried leaf and stem of Starntonia chineis DC. from Guizhou; HPLC was utilized to measure the fumaric acid content. Results: Range analysis showed that the extraction conditio on the content of fumaric acid was in the order: kinds of solvent extraction times extraction time volume ratio of solid; variance analysis indicated that kinds of solvent had greater effects than other facto. Conclusion: The optimal condition of the orthogonal test is that， the solid dissolved in 90% ethanol， extraction lasted for 2 hou， 3 times， solid to liquid volume ratio of 8 is more appropriate and the results of method investigation comply with all requirements. The technique proves to be practical， which provides theoretical foundation for measuring the content of fumaric acid in Starntonia chineis DC. [Key words] Starntonia chineis DC.; Orthogonal test; Fumaric acid; HPLC 野木瓜（starntonia chineis DC.），常用名七叶莲，为木通科（Lardizabalaceae）野木瓜属植物，性温，味微苦，具有祛风止痛及舒筋活络等功效[1-2]。临床用于各种手术止痛、坐骨神经痛、腰椎骨质增生、跌打损伤等，疗效显著，且具有无耐药性、无成瘾性、毒副作用小的特点[3-4]，是卫生部公布的药食两用植物，全株具有较高的药用价值。原标准中有一个显微鉴别，但未见该药材质量标准研究的报道。为了探究野木瓜中反丁烯二酸的含量，本实验运用高效液相色谱法测定野木瓜中反丁烯二酸的含量，并进行方法学研究，为野木瓜的质量标准研究提供一定的实验数据。 1 仪器与试药 仪器 Wate 600-2996高效液相色谱仪（美国Wate公司），Wate 2996检测器，Empower 色谱工作站；Milli-Q超纯水纯化系统（