pparγ介导的抗氧化机制在血管平滑肌细胞表型转化中作用和机制研究神经病学专业论文.docxVIP

第三军医大学博士学位论文缩略语表 第三军医大学博士学位论文 缩略语表 英文缩写 英文全称 中文名称 PPARy Peroxisome proliferator—activated receptor丫 过氧化物酶体增殖物激活受体y UCP2 Uncoupling protein 2 解偶联蛋白2 PDGF—BB Platelet derived growth factor 血小板源性生长因子．BB BSA Bovine serum albumin 牛血清白蛋白 IH Intimal hyperplasia 内膜增生 DMEM Dulbcco’S Modifed Eagle Medium 伊格尔培养基 FBS Fetal bovine serum 胎牛血清 FITC Fluorescein isothiocyanate 异硫氰酸荧光素 h Hour 小时 RSG Rosiglitazone 罗格列酮 MMP9 Matrix metalloproteinase 基质金属蛋白酶9 PCNA Proliferating cell nuclear antigen 增殖细胞核抗原 mln Minute 分钟 DHE Dihydroethidium 二氢乙锭 EEL external elastic 1amina 血管外膜 IEL intemal elastic 1amina 血管内膜 PAGE Polyacryamide gel electrophoresis 聚丙烯酰胺凝胶电泳 PMSF Phenylmethanesulfonyl fluoride 苯甲基磺酰氟 PVDF Polyvinylidene fluoride 聚偏二氟乙烯 NAC N-acetylcysteine N．乙酰半胱氨酸 ROS Reactive oxygen species 活性氧 TEMED Tetramethylethylenediamine 四甲基乙二胺 DSMO Dimethyl Sulfoxide 二甲亚枫 VSMC Vascular smooth muscle cell 血管平滑肌细胞 万方数据 第三军医大学博士学位论文PPARy 第三军医大学博士学位论文 PPARy activation impedes vascular smooth muscle cells phenotypic transformation via attenuating OXldatlVe stress Abstract Background and Obj ectives： Increasing evidence showed that abnormal proliferation and migration of vascular smooth muscle cells(VSMCs)are common event in the pathophysiology of many vascular diseases，including atherosclerosis and restenosis after angioplasty．In the healthy blood vessel，VSMCs reside within the media and remain quiescent．In resp onse to atherogenic factors，VSMCs proliferate and migrate from the media to the intima，which ultimately leads to intimal hyperplasia(IH)and atherosclerosis．Data from animal studies showed that inhibiting proliferation and migration of VSMCs could attenuate IH significantly．Among the underlying mechanisms，oxidative stress is one of the principal contributors to the proliferation and migration of VSMCs． Oxidative stress is defined as an imbalance between endogenous antioxidants and oxidants in favor of the later．Oxidative stress occurs as a result of persistent production of reactive oxygen spec