转基因油菜籽中重金属铅、镉、铜、铬、铁含量的测定.PDFVIP

※分析检测 食品科学 2008, Vol. 29, No. 08 553 魏 行，沈 敏，李胜清，薛爱芳，陈 浩 * (华中农业大学理学院化学系，湖北 武汉 430070) ：本实验对抗除草剂品种的转基因油菜籽进行湿法消解预处理，处理液采用石墨炉和火焰原子吸收光谱法 进行检测，测定重金属铅、镉、铜、铬、铁的含量，并做加标回收实验。按照相同方法处理非转基因油菜籽， 并测定其重金属的含量，通过比较，发现该品种的转基因油菜籽与非转基因油菜籽中这五种重金属的含量没有明显 差别 。 ：转基因油菜籽；重金属；原子吸收光谱法 Determination of Pb, Cd, Cu, Cr and Fe in Transgenic Rapeseeds by Atomic Absorption Spectrometry WEI Xing, SHEN Min, LI Sheng-qing, XUE Ai-fang, CHEN Hao* (Department of Chemistry, College of Science, Huazhong Agricultural University, Wuhan 430070, China) Abstract：A simple procedure was developed to determine contents of heavy metals in transgenic rapeseeds. After wet digestion of samples, five heavy metals, Such as Pb, Cd, Cu, Cr and Fe were determined by flame atomic absorption spectrometry (FAAS) or graphite furnace atomic absorption spectrometry (GFAAS) with regard to their concentrations, respectively. The experi- ments of standard addition were carried out to validate the proposed method, and good recoveries are present. According to the obtained concentrations of Pb, Cd, Cu, Cr and Fe in the transgenic rapeseeds and the non-transgenic rapeseeds, a conclusion can be made that there is no obvious difference in the contents of heavy metals between the two types of tested samples. Key words ：transgenic rapeseed ；heavy metals ；atomic absorption spectrometry 中图分类号：TS207.5　　　　　　 　文献标识码：A 文章编号：1002-6630(2008)08-0553-04 随着对转基因食品研究的不断深入，人们逐渐认识 传转化技术也已经比较成熟[6] 。油菜籽可以通过菜籽油 到转基因食品是一把“双刃剑”，在带来巨大经济效 参与人的食物链，也就是说油菜籽中的重金属也会进入 益的同时，也带来一些负面问题，尤其是人们日益关 人体并有可能蓄积。国内外学者曾对非转基因的油菜籽 注的食品安全问题[1-2] 。转基因食品中重金属含量的检测 进行过研究[7] ，但有关转基因油菜籽中重金属含量的研 及影响也逐渐引起人们的关注。Diopan