α-硫辛酸对帕金森病模型大鼠黑质铁沉积的干预机制研究-神经病学专业论文.docxVIP

万方数据 万方数据 DMT1的表达明显下调，使Ferritin、FP1的表达明显上调，减少中脑神经细胞的 铁的摄取，增加铁储存和加大铁的出胞作用，维持铁平衡的稳态。 【关键词】帕金森病；α-硫辛酸；二价金属离子转运蛋白1；细胞膜铁转运 蛋白1；铁蛋白 III Mechanism of alpha lipoic acid intervening on iron deposition in the substantia nigra of Parkinsons disease model rat Major: Neurology Postgraduate: XuLi Supervisor: Jiao Ling [Abstract] Objective: To explore the molecular mechanism of α-lipoic acid (LA)scavenging the midbrain SN iron accumulation, through observing the expressions of divalent metal transport 1 (DMT1), Ferroportin 1(FP1), and Ferritin in the midbrain SN of the model rats with 6-OHDA induced Parkinsons disease.Methods: 130 male SD rats were randomly divided into 5 groups: Control Group of 30 rats (with intracranial injection of saline solution), then 20 rats out of the Control Group were selected randomly as Sham operated Group after four weeks; Model Group of 100 rats (with intracranial injection of 6-OHDA ), four weeks later 80 rats out of Model Group were randomly divided into 4 groups:the PD Model Group (20 rats, injection of nothing) and Low Agent Group (20 rats, injection of LA), Medium Agent Group(20 rats, injection of LA), and High Agent Group (20 rats, injection of LA). Unilateral intrastriatal 6-OHDA injection (right side) was performed on anesthetized rats using stereotaxic apparatus. Control Group received a single injection of 0.9% saline. Low Agent Group received a single injection of 25mg/kg/d of LA, Medium Agent Group was injected 50mg/kg/d of LA, and High Agent Groups was injected 100mg/kg/d of LA for 14 consecutive days. After the treatment, the right midbrain SN of the rats in all groups were taken for the examination to the expression levels of DMT1, FPl, and Ferritin by Western-Blot. The number of the iron positive cells within SN were counted by the Prussian blue iron stain method. In addition, the iron contents of SN was performed using the atomic absorption spectrophotometer.Results: (1) Compared to the Sham operated Group, the expression levels of