α-硫辛酸对糖尿病大鼠肠道痛觉敏感性的影响及其分子机制的研究-内分泌与代谢病学专业论文.docxVIP

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α-硫辛酸对糖尿病大鼠肠道痛觉敏感性的影响及其分子机制的研究-内分泌与代谢病学专业论文.docx

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α-硫辛酸对糖尿病大鼠肠道痛觉敏感性的影响及其分子机制的研究-内分泌与代谢病学专业论文

PAGE IV PAGE IV 中文摘要 α-硫辛酸对糖尿病大鼠肠道痛觉敏感性的影响及其分子机制的研究结论 STZ 诱导的糖尿病大鼠肠道特异性 DRG 节神经元细胞中 P2X3 受体的表达量显著上调，细胞兴奋性增加，介导了糖尿病大鼠肠道痛觉过敏。抗氧化剂α- 硫辛酸能从分子水平阻断上述病理过程，表明氧化应激机制参与调控肠道特异性 DRG 神经元细胞中 P2X3 受体的表达及功能。关键词：糖尿病胃肠功能紊乱；肠道痛敏；背根神经节；P2X3 受体；α-硫辛酸作者：秦欣指导教师：胡吉教授 α-硫辛酸对糖尿病大鼠肠道痛觉敏感性的影响及其分子机制的研究英文摘要 Study of the intestinal hypersensitivity influenced by alpha lipoic acid and its molecular mechanism Background and Aims: In recent years, the incidence of diabetes mellitus increased year by year. Abdominal pain is one of the most common symptoms in diabetes patients.Many studies show that P2X3 receptor is closely related with sense of pain. Alpha lipoic acid is a antioxidant.It was reported that alpha lipoic acid has certain effects on diabetic peripheral neuropathy.So the study aims to explore:1.Preliminarily clarify the change of gut sensitivity in adult diabetes rats induced by STZ and the characteristics of expression of P2X3 receptor in spinal cord dorsal root ganglion (DRG) neurons.2.Study the effect of Alpha lipoic acid (ALA) on gut sensitivity in adult diabetes rats and characteristics of expression of P2X3 receptor. Methods: Adult female SD rats were selected to experiment.Diabetes rats models were induced by intraperitoneal injection of STZ at the concentration of 65 mg/kg.SD rats were randomly divided into control group (CON), diabetes group (STZ), saline treatment group (STZ + NS) and alpha lipoic acid treatment group (STZ + ALA).Using abdominal withdrawal reflex (AWR) score to assess the pain response of rats under different colon expansion pressure arrange from 20mmHg to 80 mmHg. DiI fluorochrome was injected into the colon intestinal wall.After the DRG neurons were retrograde fluorescence labelled,patch clamp technique was used to test the excitability of DRG neurons and ATP current density. Western Blotting was used to detect the expression of P2X3 receptor in the colon-asociated DRG neurons which were from diabetic rats. Result: One week after intraperitoneal inje