孕激素受体膜成分1在子宫内膜癌中表达及对细胞增殖影响.docVIP

孕激素受体膜成分1在子宫内膜癌中表达及对细胞增殖影响 　　【摘要】目的：检测子宫内膜癌组织中孕激素受体膜成分1（PGRMC1）的蛋白表达特点，分析其临床意义；并探讨PGRMC1对子宫内膜癌细胞增殖调控的影响。方法：采用蛋白质免疫印迹法（Western blot）检测70例子宫内膜癌组织及28例对照子宫内膜组织中PGRMC1蛋白表达水平；应用细胞免疫组化方法检测三种人子宫内膜癌细胞系中PGRMC1的表达定位；将PGRMC1的小分子抑制剂Ag205以不同浓度分别刺激人子宫内膜癌细胞Ishikawa、Hec-1A、KLE 72小时，应用CCK-8（cell counting kit 8）方法检测Ag205对细胞活力的影响。结果：子宫内膜癌组织中PGRMC1表达较正常内膜显著升高（P=0.038）；PGRMC1在各期子宫内膜癌组织中的表达无差异，但在Ⅰ期患者中，PGRMC1在Ⅰa期表达较Ⅰb期显著高（P=0.019）；PGRMC1表达与子宫内膜癌组织中ER表达水平相关（P=0.015），但与分化程度、淋巴结转移及脉管癌栓无关，与 PR、p53、Ki-67及bcl-2表达亦无显著相关性；20μM Ag205显著抑制三种子宫内膜癌细胞的增殖活力，其中以KLE细胞的抑制作用最明显，从最低浓度（5μM）即出现抑制效应，并随浓度增加抑制作用逐步增强。结论：子宫内膜癌内膜中PGRMC1表达显著增高；增高的PGRMC1参与调控子宫内膜癌细胞的增殖活性。 　　【关键词】子宫内膜癌；孕激素受体膜成分1；增殖活力；蛋白质免疫印迹法 　　The expression of PGRMC1 in endometrial cancer andits effect on endometrial cancer cell proliferationYU Li， ZENG Zhen， LI Ting， LIAO Qinping△. Department of Obstetrics and Gynecology， Peking University First Hospital， Beijing 100034， China 　　【Abstract】Objectives： To investigate the expression pattern of progesterone receptor membrane component 1 （PGRMC1） in endometrial carcinoma （EC）， and evaluate the probable role of PGRMC1 in the proliferation of endometrial cancer cells. Methods： 70 endometrial cancer tissues and 28 noncancerous endometrial tissues were collected as experimental group and control group respectively. The expression of PGRMC1 was detected by Western blot. Three endometrial cancer cell lines were cultured in vitro， then the expression location of PGRMC1 were tested by immunohistochemistry. The cell viability was detected by CCK-8 after treatment with Ag205 for 72 hours individually， which was the specific inhibitor of PGRMC1. Results： The expression of PGRMC1 was significantly higher in EC patients than control group （P=0.038）. There was no difference among different stages， but the expression of PGRMC1 was significantly higher in patients with stage Ia than stage Ib （P=0.019）. In endometrial carcinoma the increase of PGRMC1 was detected in those with high ER status （P=0.015）. PGRMC1 expressi