BLOOD SMEAR PREPARATION AND STAINING ustin …血涂片制备及染色奥斯汀….docVIP

BLOOD SMEAR PREPARATION AND STAINING LAB OBJECTIVE The student will prepare at least five slide smears which are even, smooth and have an acceptable feathered edge. The student will stain two of the above smears with Wright’s stain so that all formed elements are readily identifiable according to criteria outlined in the textbook. PRINCIPLE OF SMEAR PREPARATION Smear Preparation A small drop of blood is placed near the frosted end of a clean glass slide. A second slide is used as a spreader. The blood is streaked in a thin film over the slide. The slide is allowed to air-dry and is then stained. SPECIMEN EDTA anticoagulated blood is preferred. Blood smears can also be made from fingerstick blood directly onto a slide. REAGENTS, EQUIPMENT. AND SUPPLIES Glass slides, 3 x 1 inch with frosted edge Stopper piercer PROCEDURE Three methods may be used to make blood smears: 1) the cover glass smear, 2) the wedge smear and 3) the spun smear. The spun smear requires an automatic slide spinner. For the purpose of this lab exercise, we will use the wedge smear. Using the stopper piercer, place a drop of blood, about 2 mm in diameter approximately inch from the frosted area of the slide. Place the slide on a flat surface, and hold the narrow side of the nonfrosted edge between your left thumb and forefinger. With your right hand, place the smooth clean edge of a second (spreader) slide on the specimen slide, just in front of the blood drop. Hold the spreader slide at a 30 angle, and draw it back against the drop of blood. Allow the blood to spread almost to the edges of the slide. Push the spread forward with one light, smooth, and fluid motion. A thin film of blood in the shape of a bullet with a feathered edge will remain on the slide. Label the frosted edge with patient name, ID# and date. Allow the blood film to air-dry completely before staining. (Do not blow to dry. The moisture from your breath will cause RBC artifact.) PROCEDURE NOTES A good blood f