野生型vhl基因提高肾细胞癌细胞786-o对trail敏感性的实验研究-experimental study on wild type vhl gene increasing sensitivity of renal cell carcinoma 786 - o to trail.docxVIP

下载本文档

21
0
约6.89万字
约 76页
2018-06-03 发布于上海
举报
版权申诉

野生型vhl基因提高肾细胞癌细胞786-o对trail敏感性的实验研究-experimental study on wild type vhl gene increasing sensitivity of renal cell carcinoma 786 - o to trail.docx

1、有哪些信誉好的足球投注网站（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

野生型vhl基因提高肾细胞癌细胞786-o对trail敏感性的实验研究-experimental study on wild type vhl gene increasing sensitivity of renal cell carcinoma 786 - o to trail

目录摘要························································································1第一部分摘要·······················································································1第二部分摘要·······················································································3Abstract4正文第一部分野生型VHL基因提高肾细胞癌细胞786-O对TRAIL敏感性的基础研究序言································································································7材料与方法····························································································9实验结果·····························································································26讨论································································································33结论和展望··························································································46参考文献·····························································································48第二部分综述···································································································59参考文献·····························································································64附录1攻读博士学位期间发表的论文目录······································70附录2主要英文缩略词表·······························································72致谢······························································································74中文摘要第一部分实验研究野生型VHL基因提高肾细胞癌细胞株786-O对TRAIL敏感性的基础研究目的：91%的散发性肾细胞癌患者中存在肿瘤抑制基因vonHippel-Lindau(VHL)的突变或失活。VHL基因以及翻译的产物pVHL能够在转录和翻译水平调节多种与肾细胞癌发生发展相关基因的表达，但与TRAIL介导的肿瘤细胞凋亡之间的关系并不明确，本次研究的目标是在肾细胞癌786-O细胞株上明确野生型VHL基因在TRAIL介导的肿瘤细胞凋亡中的作用。方法：我们选用肾细胞癌786-O细胞株、单克隆携带野生型VHL基因全长DNA的786-O/VHL细胞以及单克隆携带VHL基因不同类型突变DNA的786-O细胞，采用不同浓度的人重组TRAIL和40ug/ml的TRAIL作用不同的时间，利用MTT和流式细胞术来检测上述细胞株对TRAIL介导肿瘤细胞凋亡的敏感性以及野生型VHL基因是否具有提高786-O细胞对TRAIL敏感性的能力。RT-PCR和Western-Blot用于分析死亡受体DR4、DR5以及凋亡抑制蛋白c-FLIL）在转录和翻译的表达水平。为了准确阐明c-FLIP(L)和TRAIL介导786-O/VHL细胞凋亡的相关性，我们用脂质体2000将c-FLIP（L）转染到对TRAIL敏感的786-O/VHL细胞中，观察高表达的c-FLIP（L）和TRAIL敏感性的关系。同时我们利用DR4和DR5的拮抗抗体来观察两种死亡受体和野生型VHL基因的关系。结果：MTT检测