血小板膜糖蛋白gpⅰbα、gpⅱbⅲa及p-选择素在肺癌患者外周血中的表达-expression of platelet membrane glycoproteins gp ⅰ b α, gp ⅱ b ⅲ a and p - selectin in peripheral blood of patients with lung cancer.docxVIP

血小板膜糖蛋白 GP Ibα、GP IIb/IIIa 及 P-选择素在肺癌患者外周血中的表达 英文摘要The Peripheral Blood Expression Of Platelet GP IbαGP IIb/IIIa And P-Selectin In Lung CancerAbstractObjective: Metastasis is the main cause of cancer-related death. The research shows that the platelets are involved in the distant metastasis of tumor cells, and the interactions of the adhesive molecules on the platelets and the ligands on the tumor cells play an important role in the the process of tumor metastasis. So our aim is to investigate theperipheral blood expression of platelet GP Ibα、GP IIb/IIIa and P-Selectin in patients withlung cancer, and to investigate the functions of the platelets, which can provide some help to impede tomor cells dissemination.Method: The platelet-rich plasma was isolated from patients with lung cancer and normal volunteers in vitro, and then the expression of platelet GP Ibα、GP IIb/IIIa and P-Selectin were measured by Flow Cytometry. The expression of normal volunteers platelet GP Ibα 、GP IIb/IIIa and P-Selectin stimulated by the platelet-poor plasma of patients with lung cancer were measured by Flow Cytometry and Western Blot. Plateletaggregation was induced by the addition of ADP or Collagen and measured by a turbidometric platelet aggregometer at 37°C with a stirring speed of 1000 rpm.Results: The expression of platelet GP Ibα、GP IIb/IIIa and P-Selectin was increased in patients with lung cancer as compared with normal volunteers. The mean fluorescenceintensity of the platelet adesive molecules of the patients with lung cancer were GP Ibα(13.94±2.69) 、 GP IIb/IIIa(7.67±1.89) 、 P-Selectin(2.84±0.60), while the mean fluorescence intensity of the platelet adesive molecules of the normal volunteers were GPIbα(7.92±2.12) 、 GP IIb/IIIa(5.21±0.58) 、 P-Selectin(1.42±0.24). When the normalvolunteers platelets were stimulated by the platelet-poor plasma of cancer patients, and we found that the surface expression of platelet GP Ibα、GP IIb/IIIa、P-Selectin and the content of GP Ibα wer