爆 炸伤创面IL1、IL6、TNFα、CRP的测定.docVIP

　　爆炸伤创面IL1、IL6、TNFα、CRP的测定 【摘要】 ［目的］检测兔肢体爆炸伤创面组织IL-1、IL-6、TNF-α和CRP含量。［方法］新西兰大白兔24只，随机分为炸伤后即刻取材组(A组，n=12)和炸后1 h取材组(B组，n=12)；0.9 g铜壳单质猛黑索金炸药(RDX)以海绵间隔5 cm，绑于左下肢股部中段前外侧，电引爆，分别取爆炸中心区(区)、爆炸边缘区(区)、爆震区(区)的肌肉组织，测IL-1、IL-6、TNF-α、CRP的含量并取对侧肢体肌肉组织做对照研究。［结果］炸伤后两组各区标本IL-1、IL-6、TNF-α、CRP含量均比正常组织高，差异显著(Plt;0.05)，且从区、区到区，含量依次降低，差异显著(Plt;0.05)：A组：区与区的IL-1含量差别有显著意义(Plt;0.05)。区与区、区与区的IL-6含量差别有极显著意义(Plt;0.01)。区与区的TNF-α、CRP含量差别有极显著意义(Plt;0.01)，区与区、区与区的含量差别有显著意义(Plt;0.05)。其余各区的含量无统计学差异(Pgt;0.05)；B组：区与区的II-1、IL-6、TNF-α含量差别有显著意义(Plt;0.05)。区与区、区与区、区与区的CRP含量差别有显著意义(Plt;0.05)。其余各区的含量无统计学差异(Pgt;0.05)。A、B两组比较，炸后组织中上述因子表达虽有增强，但差异无显著意义(Pgt;0.05)。［结论］肢体爆炸伤1 h内，创面组织中IL-1、IL-6、TNF-α、CRP表达增强，不同区域间表达有差异。 【关键词】 爆炸伤；创面；IL-1；IL-6；TNF-α；ＣRP 　　Detection of IL-1、IL-6、TNF-α and CRP in explosive wound 　　Abstract：［Objective］To detect the IL-6, IL-1, TNF-α, and CRP of rabbit’s limb tissue in the explosive wound.［Method］Twenty-four New Zealand white rabbits were randomly devided into group A and group B.The group A was taken the tissue from the explosive wound area immediately. The group B was taken the tissue from wound area after explosive wound were bend up for 1 hour.0.9 g pure cuprum stronge detonator which had a 5 cm interval with sponge was fastened on the forward and outside of the left limb of the rabbits and ignited with electic impact. The IL-1, IL-6, TNF-α and CRP of the muscle tissue which were center zone (area Ⅰ), marginal zone (area Ⅱ), knocking zone (area Ⅲ) from wound area were detected in groupA and B.The normal muscle of opposite side was controlly studied.［Result］In groupA and B, the contents of IL-1, IL-6, TNF-α, CRP were increased significantly (Plt;0.05) in each zone. Group A:the content of IL-1 had significant difference between Ⅱ zone and Ⅲ zone (Plt;0.05).The content of IL-6 had significant difference between Ⅱ zone and Ⅲ zone,Ⅰ zone and Ⅲ zone (Plt;0.01). The content of CRP, TNF-α had significant difference between Ⅰ zone and Ⅲ zone (Plt;0.05). Group B:the content of IL-1, IL-6,