il23在鼻息肉发病中作用的初步研究-preliminary study on the role of il23 in the pathogenesis of nasal polyps.docx

选取符合纳入标准的汕头大学医学院第一附属医院耳鼻咽喉头颈外科住院鼻息肉患者20 例作为实验组，采集手术切除标本 20 份；选取同时行下鼻甲部分切除术的鼻中隔矫正术住院患者 10 例作为对照组，采集下鼻甲粘膜组织标本 10 份。所有标本按要求处理后进 行下列实验。1. 组织经过匀浆后取上清，用 ELISA 的方法测定鼻息肉组与下鼻甲组的 IL-23 及 IFN- γ蛋白含量。2. 用免疫组化法检测鼻息肉组与下鼻甲组中 Stat3 的表达情况。结果1. 鼻息肉组中 IL-23 蛋白表达低于下鼻甲组，差别有统计学意义（P0.05）。2. 鼻息肉组中 IFN-γ蛋白表达与下鼻甲组中的差异无统计学意义（P0.05）。3. 鼻息肉组中 Stat3 的表达高于下鼻甲组，差别有统计学意义（P0.05），Stat3 主 要在炎症细胞、腺体胞浆中表达，部分在胞核中表达。结论1. IL-23 作为 Th1 细胞因子中的一员，在某些致病因素作用下，可以改变鼻腔微环境 中 Th1/Th2 细胞因子的比例而对鼻息肉的发病起作用。2. IL-23 可能通过调节 JAK/STAT3 信号传导途径，而促进抑制凋亡因子 Bcl-2 和 Bcl-xl 的过度表达，引起嗜酸性粒细胞的积聚、浸润，最终导致息肉形成。3. IL-23 可能作用于嗜酸性粒细胞，促进其凋亡，当 IL-23 减少，则使嗜酸性粒细胞 凋亡延迟，而导致嗜酸性粒细胞的浸润、积聚，最终导致鼻息肉的形成。4. IFN-γ在东西方人鼻息肉组织中的差异表达，值得进一步关注。关键词白细胞介素-23；信号传导与转录激活因子 3；鼻息肉；嗜酸性粒细胞；凋亡AbstractBackground and ObjectNasal polyp is a disease of chronic nasal mucous membrane-nasal sinuses mucous membrane inflammation, as a very common disease of human respiratory tract, it is a very severe threaten to human health and brings down patient’s quality of life.A lot of articles reported the pathogenesis of chronic nasal mucous membranenasal sinuses mucous membrane inflammation is very complicated and unclear,so exact etiology and pathogenesis of nasal polyp are still under debate.In the past 10 years,the role of cytokines in the pathogenesis of nasal polyp is very arrestive. Recently,A lot of articles reported the pathogenesis of nasal polyp was an overbalancing course of cell proliferation and apoptosis, and some researchers reported the abnormal apoptosis of eosinophil along with the abnormal expression of much cytokines in nasal polyp.Therefore,regulated the chronic inflammation of nasal polyp by modulateing eosinophil apoptosis and accommodateing the Cytokine in the micro-environment of nasal cavity has become the new field to discover nasal polyp’s pathogenesis.IL-23，discovered by Oppmann B in 2000,is a new member of Cytokines family of IL-12.Lots of researchs indicated it had a similar bioactivity with IL-12,both of them play an important role in early inflammation. A