il18基因启动子区137cg位点多态性与2型糖尿病肾病的相关性分析-correlation analysis between polymorphism of 137 cg site in promoter region of il18 gene and type 2 diabetic nephropathy.docx

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il18基因启动子区137cg位点多态性与2型糖尿病肾病的相关性分析-correlation analysis between polymorphism of 137 cg site in promoter region of il18 gene and type 2 diabetic nephropathy.docx

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il18基因启动子区137cg位点多态性与2型糖尿病肾病的相关性分析-correlation analysis between polymorphism of 137 cg site in promoter region of il18 gene and type 2 diabetic nephropathy

关键词：白细胞介素-18；基因多态性（SNP）；2型糖尿病；糖尿病肾病AssociationstudyofSNP-137C/GinInterleukin-18genepromoterandPatientswithType2DiabeticNephropathyABSTRACTObjective:Tostudythedistributionofinterleukin-18(IL-18)genepromotersequence-137C/G，thesinglenucleotidepolymorphismgenotypesandallelefrequenciesinGuangxi-guibeiHanspopulationofChina，andtoanalyzetheassociationwithtype2diabeticnephropathy.Methods:Polymerasechainreaction-restrictionfragmentlengthPolymorphism(PCR-RFLP)analysiswasusedtodetectIL-18gene-137C/GpolymorphisminHanspopulationinGuangxi-guibeiregion.Thesubjuctswere58unrelatedpatientswithdiabetesmellitus(DMgroup)，50diabeticnephropathy(DNgroup)and40normalcontrols.Atthesametime,plasmalipids,lipoproteinlevels,includingserumtotalcholesterol(TC),triglyceride(TG),highdensitylipoprotein-cholesterol(HDL-C),lowdensitylipoprotein-cholesterol(LDL-C),apolipoproteinA1(apoAI),apolipoproteinB(apoB)wasmeasuredusingautomaticbiochemicaltests.Results:IL-18gene-137C/GlocusCC,GC,GGgenotypespresentinHanspopulationofGuangxi-guibei.TheCCgenetypesandCallelefrequencydistributionsofSNP-137werehigherinDNpatientswithoutDNthanthatinhealthycontrols(P＜0.05，OR=1.635，95%CI：0.978～2.732）.ThedistributionofgenetypesandallelefrequenciesofSNP-137polymorphismhadstatisticaldifferencebetweenT2DMpatientswithDN.DiabeticnephropathypatientswithTC,TGandLDL-Clevelwassignificantlydifferent(P0.05).Conclusions:ThereisIL-18-137C/GsinglenucleotidesitepolymorphisminHanspopulationofGuangxi-guibei.Itsdistributionareobviousdifferencesamongdifferentethnicgroups.IL-18gene-137G/Cpolymorphismanddiabeticnephropathywasassociated,CallelemaybeageneticriskfactorincidenceofDN.ThepolymorphismmaybeahighdegreebypromotingtheexpressionofIL-18increasedriskofDN.KeyWords:Interleukin-18；singlenucleotidepolymorphism;type2diabetes;diabeticnephropathy英汉缩略词对照表英文缩写英文全称中文全称IL-18Interleukin-18白细胞介素18IL-18RInterleukin-18Receptor白细胞介素18受体IL-12Interleukin-12白细胞介素12DNADeoxynucleieacid脱氧核糖核酸脱氧核糖核昔三磷dNTPDeoxynucleosidestriphosphat酸盐IFN-γInterferon-γγ－干扰素ECMExtracellul