hsamir223在人类围着床窗期子宫内膜的表达及调控机制-expression and regulatory mechanism of h samir 223 in human endometrium around the bed window.docx

英文缩略词表英文缩写ART英文全称Assisted reproductive technique中文名称辅助生殖技术miRNALIFmicroRNALeukemia inhibitory factor微小 RNA白血病抑制因子UTRUntranslated Regions非翻译区COX-2 TGF-βCyclooxygenase-2 Transforming growth factor-beta环氧合酶-2 转化生长因子-betaESCEndometrial stromal cell子宫内膜基质细胞DEPCDiethypyrocarbonate焦碳酸二乙酯mgMilligram毫克LLiter升mlMilliliter毫升ulmicro liter微升hHour小时minMinute分钟sec, sSecond秒rpmrevolutions per minute每分钟转速bpbase pair碱基对KDKilo Dalton千道尔顿Hsa-miR-223 在人类围着床窗期子宫内膜的表达及调控机制学位申请人：黄凯导师：章汉旺教授摘要目的：探讨 has-miR-223 和 LIF 在围着床期子宫内膜的表达规律以及二者调控胚胎着 床的相关性。 方法：收集因男性不育或输卵管因素继发不孕的患者增生晚期及分泌中期子宫内膜各 6 例，利用 Real time PCR 检测 has-miR-223 在两组子宫内膜中的表达差异。免疫组化 定位 LIF 蛋白在两组子宫内膜中的表达位置及强度。RT-PCR 和 Western Blot 分别在 mRNA 水平和蛋白水平检测 LIF 在两组子宫内膜中的表达差异。结果：1、hsa-miR223 在人增生晚期子宫内膜的表达明显高于分泌中期。2、LIF 蛋白 主要表达在子宫内膜的腺上皮细胞，基质细胞中也有一定量的表达。3、与增生晚期 子宫内膜相比，分泌中期子宫内膜 LIF 的表达在 mRNA 水平和蛋白水平均明显升高， 差异均具有统计学意义。结论：has-miR-223 可能通过调控人子宫内膜中 LIF 的表达在胚胎着床中发挥重要作 用。关键词：has-miR-223；LIF；围着床期；胚胎着床本课题受到国家自然科学基金“miRNA223/1237调控胚胎着床的功能与机制研 究”（NO的资助。The Expression and Regulation Mechanism of hsa-miR-223 in human periimplantation endometriumCandidate: Kai Huang Supervisor: Hanwang ZhangABSTRACTObjective: To investigate the expression of hsa-miR-223 and LIF in human periimplantation endometrium and explore the relationship of them in regulating embryonic implantation.Methods: six late proliferative endometria and six mid-secretory endometria was obtained from patients of secondary infertility caused by male factor or tube factor, respectively. Real time PCR was used to detect the expression of hsa-miR-223 in two groups. Immunohistochemical staining was applied to detect the location and expression level of LIF protein. RT-PCR and Western Blot was used to observe the expression of LIF in mRNA and protein level.Results: 1. The expression of hsa-miR-223 in late proliferative endometria was significant higher than that in mid-secretory endometria. 2. LIF protein was observed in both endometrial epithelial cells and stromal cells, mainly in epithelial