hpv负荷量和基因型检测在子宫颈病变中临床意义-clinical significance of hpv load and genotype detection in cervical lesion.docx

[Objective]ToinvestigatetheHPVDNAloadandtoexploreinuterinecervixwithdifferentpathologicalchanges.ToinvestigatedistributionofHPVgenetypesinHefeiandtoexploretherelationshipsbetweenmultipleinfectionsanddifferentcervicallesions[Methods]HCⅡwasusedtodetectHPVDNAloadindifferentcervicallesions.TodetectdistributionofHPVgenotypesindifferentcervicallesionsbyFlow-ThroughHybridizationAndGeneChip(HybriMax).[Results]DetectionofHPVDNAloadbyHCⅡ,CINⅠ～Ⅲandcervicalcancercomparedwiththecontrolgroup,therewassignificantdifference(P0.05);andtherewasnosignificantlydifferencebetweenCINⅠ～Ⅲandcervicalcancer(p0.05).InHefei,themostcommonhighriskHPVtypeswereHPV16（50.0%）and52（26.7%）,followedby33（11.4%）,58（8.5%）and18（6.4%）;Lowrisktypes11(8.5%)and6(3.4%)wereprevalence;AmongChinesepopulartypesHPV66washigherprevalence.Singleandmultipleinfectionratewas62.7%（148/236）and37.3%（88/236）,respectively.Dualinfectionwasmaintypeofmultipleinfection,themostcommonmodelswereHPV16/52combinedwithothertypes.CINⅡ～Ⅲmultipleinfectionratewashighercomparedtonegativeorcervix、CINⅠandcervicalcance（rP0.05）.Sigleinfectionwasmorecommonincervicalcancer.[Conclusion]HPVDNAloadlevelsdoesnotmeanthattheseverityofthedisease,however,thehigherHPVDNAloadthegreaterriskofCINandcervicalcancer.HPV16,52,18,33and58arethemostprevalencehigh-risksubtypes,inHefei.DoubleinfectionespeciallyHPV16/52inconjunctionwithothersubtypesareassociatedwithCINⅡ～Ⅲ,butnotcervicalcancer.DetectionofHPVDNAloadandgenotypeislikelytobetoimproveearlydiagnosisofcervicalcancerandprovidemoreeffectiveandmorereasonableprogram,andmaybeasamonitoringandevaluatingindicatorsofcervicaldiseaseprognosis.[Keyword]HPV/genetype；viralload；CIN；cervicalcancer安徽医科大学硕士学位论文英文缩略词表英文缩写CIN英文全名cervicalintraepithelialneoplasia中文全名宫颈上皮内瘤变ASCUSAtypicalSquamousCellsof无明确诊断意义的非典型鳞状UndeterminedSignification上皮细胞LSILLigh-gradesquamousintraepithelial低度鳞状上皮内病变HSILHigh-gradesquamousintraepithelial高度鳞状上皮内病变neoplasiaLCTLiquidThinprepCytologyTest液基细胞学检查RNARibonucleicacid核糖核酸hTERThumantelomerasereverse端粒酶逆转录酶tran