hmgb1 nfκb与炎性复合体在急性白血病患者中的表达及意义-expression and significance of hmgb1 nf κ b and inflammatory complex in patients with acute leukemia.docx

下载文档 降价啦

3
0
约4.34万字
约 51页
2018-05-29 发布于上海
举报
版权申诉
保障服务

hmgb1 nfκb与炎性复合体在急性白血病患者中的表达及意义-expression and significance of hmgb1 nf κ b and inflammatory complex in patients with acute leukemia.docx

1、本文档共51页，可阅读全部内容。
2、有哪些信誉好的足球投注网站（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。

hmgb1 nfκb与炎性复合体在急性白血病患者中的表达及意义-expression and significance of hmgb1 nf κ b and inflammatory complex in patients with acute leukemia

ABSTRACTObjective:ToexploreHMGB1、NF-κBandInflammasomeNLRP3、ASCandAIM2expressionlevelsandtherelationshipwiththeoccurrence，developmentandprognosisinpatientswithacuteleukemia，itmaybeprovidelaboratoryevidencefordiagnosis、treatmentandrecurrencepredictionofacuteleukemia.Methods:Wecollectedthebloodandbonemarrowsamplesfrom18casesofALLand42casesofANLLatotalof60patientswithacuteleukemia(experimentalgroup)and20patientswithoutmalignancedisease(controlgroup)intheHematologyDepartmentoftheFirstAffiliatedHospitalofGannanMedicalCollegefromJuly2013toJuly2014.Theexperimentalgroupwasdividedintountreatedgroup、recrudescentgroupandcompleteremissiongroup,eachgroupwas20cases.ELISAdetectHMGB1、NLRP3、ASCandAIM2antigenlevelinplasma,RT-PCRdetectHMGB1andNF-κBgenelevelinbonemarrowmononuclearcells,WesternblotdetectHMGB1andNF-κBproteinlevelinbonemarrowmononuclearcells，IHCdetectHMGB1andNF-κBexpressionlevelinbonemarrow.Results:ELISAshowedthatHMGB1antigenlevelinuntreatedgroupandrecrudescentgroupweresignificantlyhigherthanincontrolgroup（P＜0.05）,NLRP3、ASC、AIM2antigenleveldoingtheopposite,therewasnostatisticallysignificantdifferencebetweencompleteremissiongroupandcontrolgroup（P＞0.05）;andtherewasnostatisticallysignificantdifferencebetweenALLandANLL（P＞0.05）.RT-PCRshowedthatHMGB1andNF-κBgeneexpressionweresignificantlyincreasedinuntreatedgroupandrecrudes-centgroupcomparedtocontrolgroup（P＜0.05）,butnotinthecompleteremissiongroup（P＞0.05）,therewasnostatisticallysignificantdifferencebetweenALLandANLL（P＞0.05）.WesternblotshowedthatHMGB1andNF-κBproteinexpressionlevelsweresignificantlyelevatedinuntreatedgroupandrecrudescentgroup（P＜0.05）,butnotincompleteremissiongroup（P＞0.05）,therewasnostatisticallysignificantdifferencebetweenALLandANLL（P＞）.IHCshowedthatHMGB1andNF-κBismainlyexpressedinuntreatedgroupandrecrudescentgroup.HMGB1waspositivelycorrelatedwithNF-κB,butnegativelycorrelatedwithNLRP3、ASCandAIM2.ThesurvivaltimeofHMGB1overexpressionpatientsislonglythanthatofnon-overexpressionpatients.Conclusions:TheInflammasomeNLRP3