hl60细胞裸鼠异种移植瘤差异蛋白质的分析-analysis of differential proteins in hl60 cell xenografts in nude mice.docx

analyzed under the Swiss-Prot database annotation information, and we found that the up-regulated protein mainly located in nuclear, whereas the down-regulated protein mainly located in cytoplasm. While the main function of these proteins are divided into: binding, catalytic, transcription regulation, transport and so on. Using Western blot technique and immunohistochemistry, respectively, two up-regulated protein T- complex protein submit alpha and Heterogeneous nuclear ribonucleoprotein H1, two down-regulated protein Enolase 1 and Glutathione S-transferase P were verified, the results are better with the DIGE results consistency.In this study, proteomics technology was successfully used to identify, analyze and verify a number of differential expression proteins, which associated with increased tumorigenicity. These up-regulated or down-regulated proteins play an important part in the occurrence and development of the increase of tumorigenicity. The further study of these proteins can provide evidence to the occurrence and development of acute myeloid leukemia.Key words: HL-60; Proteomics; DIGE; Western blot英文缩写词对照表英文缩写英文名称中文名称2DETwo-dimensional gel electrophoresis二维电泳ACNAcetonitrile乙腈BSABovine serum albumin牛血清白蛋白BVABiological variation analysis生物学变异度分析CHAPS3-[(3-Cholamidopropyl)3-[3-（胆酰胺基丙基）二甲dimethylammonio] propanesulfonic acid氨基]丙磺酸盐DIADifferential in-gel analysis胶内差异分析DIGEDifferential in-gel electrophoresis差异凝胶电泳DMFDimethylformamide二甲基甲酰胺DTTDithiothreitol二硫苏糖醇EDTAEthylenediamine tetraacetic acid乙二胺四乙酸IPGImmobilized pH gradient固化 pH 梯度GAPDHGlyceraldehydes 3-phosphate dehydrogenase3-磷酸甘油醛脱氢酶GSTP1Glutathione S-ransferase P谷胱甘肽硫转移酶 πhnRNP H1Heterogeneous nuclear ribonucleoprotein H1核不均一核糖核蛋白 H1MALDIMatrix-assisted laser desorption inoization基质辅助激光解吸离子化MSMass spectrometry质谱MWMolecular weight分子量PAGEPolyacylamide gel eletrophoresis聚丙烯酰胺凝胶电泳PBSPhosphate buffered saline磷酸盐缓冲液pHPower of hydrogen酸碱度pIIsoelectric point等电点PVDFPolyvinylidene聚二氟乙烯Q-TOF MSQuadrupole-time of