用二次回归正交旋转组合设计优化桑叶多酚的提取工艺.docVIP

用二次回归正交旋转组合设计优化桑叶多酚的提取工艺 594蚕业科学CANYEKEXUE2009;35(3) 用二次回归正交旋转组合设计优化桑叶多酚的提取工艺 沈维治廖森泰刘吉平邹宇晓李妍. (华南农业大学动物科学学院,广州510642; 广东省农业科学院蚕业与农产品加工研究所,广东省农产品加工公共实验室,广州510610) 摘要桑叶多酚有消除机体内自由基毒性的作用,为桑叶中具有药用和保健功能开发价值的成分之一.运用二 次回归正交旋转组合设计法,研究了桑叶多酚提取工艺中原料溶剂乙醇的浓度,以及原料的质量浓度,原料溶液 pH值,提取次数等因素对桑叶多酚得率的影响,建立了具有良好预测性能的提取条件数学模型,确定了最优提取 工艺条件.试验结果表明,在桑叶多酚提取工艺条件各因素中,对桑叶多酚得率影响作用大小的顺序为原料溶液 pH值gt;原料的质量浓度gt;溶剂乙醇的浓度.最优提取工艺条件为:以70%乙醇作原料溶剂,原料质量浓度0.025 g/mL,原料溶液pH值4.在此优-fk~_v-艺条件下,从桑叶中提取桑叶多酚的质量比为16.11mg/g. 关键词桑叶;多酚;二次回归正交旋转组合设计;提取工艺 中图分类号S888.2文献标识码B文章编号0257—4799(2009)03—0594—05 OptimizationofPolyphenolExtractionFromMulberryLeaves UsingQuadraticRegressionRotationalCombinationalDesign SHENWei—ZhiLIAOSen—TaiLIUJi—PingZOUYu—XiaoLIYan (CollegeofAnimalScience,SouthChinaAgriculturalUniversity,Guangzhou510642,China TheSericultureamp;FlaProduceProcessingResearchInstitute.GuangdongAcademyofAgricultureSciences. KeyLaboratoryofAgro—foodScienceandTechnologyofGuangdong,Guangzhou510610,China) AbstractBeingcapableofeliminatingtoxicityoffreeradicalsinorganisms.thepolyphenolsfrOmmulberry leavesareoneofthevaluablecomponentswhichcanbedevelopedforuseofmedicinalandhealth—-carefunc-- tionsBymeansofquadraticregressionrotationalcombinationaldesign,theeffectofethan@concentration, materialmassconcentration.DHofmaterialsolutionandextractiontimesontheyieldofpolyphenolfrOmmul— berryleaveswasstudied.Amathematicalmodelofextractionconditionswithgoodpredictionperformance wasestablishedandanoptimumconditionforpolyphenolextractionwasobtainedTheresultshowedthatthe rankingofthefactorwaspHvaluegt;massconcentrationgt;ethanolconcentration.accordingtotheirinfluence ontheyieldofpolypheno1.Theoptimumconditionfortheextractionwasasfollowing:70%ethanolasextrac— tionsolvent,materialmassconcentration0025g/mL,andpH4Undersuchcondjtions,theyieldofpoly— phenolfr0mmulberryleaveswas1611mg/g KeywordsMulberryleaf;Polyphenol;Quadraticregressionrotationalcombinationdesign;Extraction Process 收稿日期:2009—03—27 资助项目:,东省科技厅社会发展攻关项目(编号2008A030101001) 广州市科技攻关项目(编号2007