8及其抑制剂TIMP-1在心肌重塑中的动态表达.docVIP

8及其抑制剂TIMP-1在心肌重塑中的动态表达 第32卷第2期 2007年4月 贵阳医学院 JOURNALOFGU1YANGMEDICALCOLLEGE V01.32No.2 20o7.4 TGF[31,MMP一8及其抑制剂TIMP一1在心肌重塑中的 动态表达 方丽,杨婷,张晓林,杨国雄,杨勤 (贵阳医学院病理生理学教研室,贵州贵阳550004) [摘要]目的:探讨基质金属蛋白酶(MMP~)及其生理性抑制剂TIMP-1和转化生长因子p1(TGF~I)在大 鼠压力负荷性心肌重塑中的动态表达及作用.方法:环扎成年Wistar大鼠腹主动脉使之缩窄,造成左心室压力 负荷引起心肌重塑.观察和比较术后2,4,8,12周及假手术组大鼠左心室肌重量指数,Masson染色后比较心肌 胶原含量,免疫组织化学SABC法及蛋白免疫印迹方法观察MMP.8,TIMP一1,TGFIM蛋白表达.结果:腹主动脉 缩窄术后各组大鼠发生左室重塑和纤维化,左心室肌重量指数,胶原含量及TGF~I蛋白表达均明显高于假手术 组;术后2,4,8周MMP.8表达均高于假手术组,但12周时降至假手术组水平;nMP一1仅在术后2周有一过性增 高.结论:TGF[31表达增多,MMP-8/nMP.1表达相对上调可能参与了大鼠压力负荷性心肌重塑的发生和发展. [关键词]心肌;心肌缺血;基质金属蛋白酶;金属蛋白酶I组织抑制剂;转化生长因子B [中图分类号]R542.23[文献标识码]A[文章编号]1000-2707(2007)02-0159-05 DynamicExpressionofTransformingGrowthFactor131(TGF[M), Metalloproteinase(MMP-8)andTissueInhibitorof Metalloproteinase(TIMP-1)inMyocardialRemodeling FANGLi,YANGTing,ZHANGXiaolin,YANGGuoxiong,YANGQin (DepartmentofJI叩蝴,GmyangMedicalCollage,Guiyang550004,China) [Abstract]Objective:Tostudytheexpressionandtheeffectsofmetalloproteinase(MMP-8),tissue inhibitorofmetalloproteinase(TIMP一1)andtransforminggrowthflac131(TGF~I)inpressureover- loadrats.Methods:MyocardialremodelingmodelofadultWistarratswasestablishedbycoarctating ofabdominalaortaandcreatingpressureloadinleftventriculartocausemyocardialremodeling.Ex— pressionofMMP-8,TIMP一1,andTGF~I,andmyocardialremodelingindexwereevaluatedinthe2, 4th. 8thand12thweeksafteroperationandshamoperation.CollagencontentsweremeasuredwithMas— sonstainedsectionsofcardiacmuscle.ExpressionofMMP一8,TIMP一1,andTGF~1wastestedwithim— munohistochemistrySABCmethodandproteinimmunoblotting.Results:Myocardialremodelingandfi— brosispredominantlyoccurredinleftventricleofratsinmodelgroups.Theleftventricularhypertrophic index,expressionofcollagenandproteinofTGFI31werehigherinratsofmodelgroupsthaninratsof shamoperationgroup(Plt;0.01).ExpressionofproteinofMMP-8elevatedinthe2Ⅱd,4chand8th weeksinratsofmodelgroups,whichwashigherthanthatinshamoperationgroup,and,then,de— creasedtothelevelofshamoperationgroupinthe12恤week(Plt;0.