广西45个主栽水稻品种抗性基因遗传多样性分析.docVIP

广西45个主栽水稻品种抗性基因遗传多样性分析 南农业2011,42(10):1173—1176 JournalofSouthernAgricuhure 广西45个主栽水稻品种抗性基因遗传多样性分析 张晋..,罗志勇,颜群,钟波,高利军,潘筱凌.,赖海燕, 黄鹂,高汉亮,郭小强 (.广西农科院植物保护研究所,南宁530007;广西作物遗传改良生物技术重点开放实验室,南宁530007 广西区种子管理总站.南宁530022) 摘要:【目的】明确近年来广西45个主要栽培水稻品种的抗性基因遗传多样性,揭示其遗传差异,为更好筛选水稻 品种组合和通过品种间轮作及合理布局控制病害提供理论依据.【方法】根据已克隆的抗病基因设计5对RGA引物,对 广西45个主栽水稻品种进行抗病基因同源序列分析并构建聚类图谱.【结果】在45个水稻品种的抗病基因中,共扩增 出813条带,其中多态性条带124条,多态率达76.07%.聚类结果显示,不同品种间遗传相似系数变幅为0.61—0.93,取相 似系数0.67时,可将45个水稻品种分为5大类,聚类结果在一定程度上反映品种来源的地域性和亲缘关系.【结论】广 西不同主栽水稻品种间抗病基因数量差异较大,在广西稻瘟病发生严重的地区,采取不同抗性遗传背景的水稻品种 轮作及混栽是控制该病害最为有效的措施之一. 关键词:水稻;主栽品种;抗病基因同源序列;遗传背景;广西 中图分类号:S511文献标志码:A文章编号:2095一l191(2011)10—1173—04 Geneticdiversityamongst45mainricecultivarsinGuangxi ZHANGJin一,LUOZhi—yong,YANQun,ZHONGBo.,GAOLi-jun一,PANXiao—ling, LAIHai—yan,HUANGLi.,GAOHan—liang,GUOXiao—qiang (PlantProtectionInstitute,GuangxiAcademyofAgricultureSciences,Nanning530007,China;GuangxiCropGenetic ImprovementandBiotechnologyLab,Nanning530007,China;GuangxiSeedsManagementStation,Nanning530022,China) Abstract:【Objective]Thepresentexperimentwasconductedtoassessthegeneticdiversityinrelationtogeneticvari— ationsindiseaseresistanceamongst45mainricecuhivarsofGuangxi.【Method】FivepairsofRGAprimersweredesigned onthebasisofalreadyidentifieddiseaseresistantgenesinordertofindoutthepresenceorabsencein45ricecultivars. 【Result]Total813bandswereamplifiedfrom45ricecultivars,ofwhich124werepolymorphicandthepolymorphicrate was76.07%.Theclusteranalysisresultshowedthatthesimilaritycoefficientofthesecuhivarsrangedfrom0.61to0.93and thesecuhivarsweredividedinto5classificationsat0.67.Italsorevealedthepresenceoffivedistinctindicatingregionalism anddistantgeneticrelationshipsamongstricecuhivarsources.【Conclusion】Significantgeneticvariationshavebeenob— servedinresistancetovariousdiseases.Cultivarrotationandplantingofmixed—cuhivarswithdifferentgeneticbackgrounds havebeensuggestedasaneffectivewayforcontrollingriceblastdiseaseinGuangxi. Keywords:rice;cuhivars;homologicalsequenceofresistantgenetodisease;ge