早期生物标记诊断术后急性肾脏衰竭.pptVIP

Sham 3 6 12-112-2 24-124-2 48-1 48-2 (hr) WB: Urinary HJV 15% SDSCBR staining Urinary HJV from rat after IR-Injury After IRI Urinary HJV after 24 hr post-operation from NSARF databank 　 　 　 　 　 1930 119 78 2 M.V.R. 320 2 60y 劉XX霞 0 1 0 1 1 1415 177 99 2 A.V.R. 394 1 57y 趙X福 0 1 0 2 1 1470 101 0 1 O.P.C.A.B 465 2 72y 潘X鑾 　 　 　 　 　 1495 165 135 2 M.V.R. 240 1 59y 楊X龍 　 　 　 　 　 2564 113 79 1 O.P.C.A.B 235 1 74y 林X鐘  yes=1 yes=1 yes=1 Cr.=1U=2 yes=1(7day) 24hr total urine Total bypass time (min) Aortic Clamp time (min) CAD=1Valve=2CABG+Valve=3Valve+Other=4Aorta diseasr=5OTHER=6 術式 手術時間 Male (M=1) Age Name 轉出入death Renal recovery ARF With dialysis RIFLE ARF Urine 　 　 　 　 　 　 　 林 楊 潘 趙 劉 HJV WB: 1st Anti-HJV pAb 15% SDSConclusion Urinary netrin-1 is an early diagnostic biomarker of AKI in post-cardiac surgery patients. Hemojuvelin may be a potential biomarker involved in iron homeostasis after IRI. * Early biomarkers for diagnosis of postoperative acute renal failure 早期生物標記診斷術後急性腎臟衰竭 楊 光 華 Devarajan P (2005) Curr Opin Pediatr Metabolic consequences of acute IRI to kidney tubule cells Sensitivity Speed Simple workflow Comprehensive profiles High multi-plexity The characteristic of biomarker Goal Develop suitable monitoring system via biomarkers from NTUH SICU ARF databank. Identify the role of AKI biomarkers among injury, protection or repair. The animal model of ischemia-reperfusion injury for AKI 40 min 3 6 12 24 48 (hr) 0 Sham The serum creatinine from post-operative rats Serum creatinine measurements obtained at various time points post-cardiac surgery from NSARF databank Urine netrin-1 from 9 AKI patients in NSARF databank 1 2 3 4 5 6 7 8 9 10 11 12 13 Netrin-1 in urine collected from 1-2: normal health volunteers, 3-10: postoperative patients without acute kidney injury (AKI), 11-13: postoperative patients with AKI. 40 mg creatin