“桂枝与白芍”药对化学成分uplc―qtof―ms分析.docVIP

“桂枝与白芍”药对化学成分UPLC―QTOF―MS分析 　　[摘要] 目的 采用液?|联用技术（UPLC-Q/TOF-MS）对“桂枝与白芍”药对中的化学成分进行定性分析。方法 采用Agilent 1290 UPLC和YMC-Triart C18（100 mm×2.1 mm，1.9 μm）色谱柱进行快速物质分离；流动相为乙腈-0.1%冰醋酸梯度洗脱；温度：30℃；进样量：5 μL；检测波长：254 nm；流速：0.3 mL/min；AB 5600质谱使用ESI离子源分别在正离子与负离子模式下采集数据。 结果 结合对照品确认了8个化合物，通过质谱信息与相关文献数据推测13个化学成分，对其进行成分归属。其中主要含有没食子酸、原儿茶酸等6种有机酸，芍药苷、芍药内酯苷等10种芍药总苷单萜类化合物及香豆素类成分。 结论 采用UPLC-Q/TOF-MS确定“桂枝与白芍”药对中的化学成分，为阐明“桂枝与白芍”药对的药效物质基础提供有力的证据。 　　[关键词] 桂枝；白芍；药对；UPLC-Q/TOF-MS；成分鉴定；物质基础 　　[中图分类号] R927 [文献标识码] A [文章编号] 1673-7210（2017）06（a）-0012-05 　　Chemical constituents analysis of the drug pair of Cinnamomum cassia Presl and Paeoniae Radix Alba by UPLC-Q/TOF-MS 　　CHEN Yongcai1 QIAN Jianghui2，3 WANG Binhui4 SHA Xianyi2，3 　　1.Department of Pharmaceutics， Hospital of TCM in Wenzhou City， Zhejiang Province， Wenzhou 325000， China； 2.Department of Pharmaceutics， School of Pharmacy， Fudan University， Shanghai 201203， China； 3.Institute of Integrated Traditional Chinese and Western Medicine， Fudan University， Shanghai 201203， China； 4.Department of Pharmaceutics， Affiliated Municipal Hospital of Taizhou Medical College， Zhejiang Province， Taizhou 318002， China 　　[Abstract] Objective To develop an ultra-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometric （UPLC-Q/TOF-MS） method to analyze the chemical constituents in the drug pair of Cinnamomum cassia Presl and Paeoniae Radix Alba. Methods Agilent 1290 UPLC machine and YMC-Triart C18 column （100 mm × 2.1 mm， 1.9 μm） were adopted to separate ingredients； the mobile phase was acetonitrile （A）-0.1% acetic acid solution （B） with gradient elution； the column temperature was 30℃； the inject volume was 5 μL； the detection wavelength was set at 254 nm； the flow rate was 0.3 mL/min； the mass spectrometer AB 5600 with an electrospray ionization （ESI） interface was under positive or negative mode for data collection. Results 8 compounds were identified by reference substance， 13 compounds were qualitative identified ac