甲氨蝶呤联合复方风湿宁对类风湿性关节炎滑膜成纤维细胞Treg／Th17免疫平衡的影响.docVIP

精品论文 参考文献 甲氨蝶呤联合复方风湿宁对类风湿性关节炎滑膜成纤维细胞Treg／Th17免疫平衡的影响 陈悦 　　（南充市中心医院风湿免疫科 四川 南充 637000） 　　【摘要】 目的：研究对类风湿性关节炎(RA)的患者采用甲氨蝶呤联合复方风湿宁治疗，对其滑膜成纤维细胞(FLS)Treg/Th17免疫平衡的影响。方法：选取本院于2014.9-2015.9期间收治的60例类风湿性关节炎的患者，按照随机数字表法将其分为A组30例与B组30例，A组采用甲氨蝶呤联合复方风湿宁治疗，B组单独使用复方风湿宁治疗，临床检测FLS增殖情况，使用MTT法检测；用ELISA法检测IL-10、IL-17、IL-22水平。观察两组OD值、FLS增殖抑制率及IL-10、IL-17、IL-22水平。结果：A组OD值低于B组，Plt;0.05。A组FLS增值抑制率高于B组，Plt;0.05。A组IL-10水平高于B组，Plt;0.05；B组IL-17、IL-22水平高于A组，Plt;0.05。结论：采用甲氨蝶呤联合复方风湿宁治疗RA，相较于单独使用复方风湿宁，可有效降低FLS中OD值，提高FLS增殖抑制率，提高IL-10水平。 　　【关键词】类风湿性关节炎；FLS；复方风湿宁 　　【中图分类号】R453 【文献标识码】A 【文章编号】2095-1752（2016）12-0097-02 　　 　　Joint compound anti-rheumatism methotrexate for rheumatoid arthritis synovial fibroblasts Treg/Th17 immune balance 　　Chen Yue 　　Nanchong central hospital rheumatism lmmunity branch Sichuan nanchong 637000,China 　　【Abstract】 Objective Research on rheumatoid arthritis (RA) patients treated with methotrexate joint compound anti-rheumatism, the synovial fibroblasts (FLS) Treg/Th17 immune balance. Methods Selection in our hospital from 2014.9 to 2015.9 admitted during the period of 60 patients with rheumatoid arthritis, according to random number table method to the 30 cases were divided into group A and group B, 30 cases in group A treated with methotrexate joint compound anti-rheumatism, group B treated with compound anti-rheumatism alone, clinical detection of FLS proliferation, determined by MTT method were used to detect; With ELISA method to detect IL - 10, IL - 17, IL - 22. Observe two groups of OD value, FLS proliferation inhibition rate and IL - 10, IL - 17, IL - 22. Results OD value lower than group B, group A (P lt; 0.05. FLS value-added inhibition rate higher than that of group B, group A (P lt; 0.05). IL - 10 group A level higher than that of group B, P lt; 0.05; Group B IL - 17 to 22, IL level higher than that of group A, P lt; 0.05). Conclusion Using methotrexate combined compound anti-rheumatism RA treatment, compared with si