2015-J Proteome Res-耐药性结核分支杆菌的第一次蛋白琥珀酰修饰图谱分析揭示了琥珀酰修饰参与了细胞生理学过程.pdfVIP

Article /jpr First Succinyl-Proteome Profiling of Extensively Drug-Resistant Mycobacterium tuberculosis Revealed Involvement of Succinylation in Cellular Physiology Longxiang Xie,⊥,† Wei Liu,⊥,‡,§ Qiming Li,† Shudan Chen,∥ Mengmeng Xu,† Qinqin Huang,† Jie Zeng, † Mingliang Zhou,† and Jianping Xie*,† †Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Southwest University, Beibei, Chongqing 400715, China ‡Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai 200040, China §Hangzhou Center for Disease Control and Prevention, Binjiang, Hangzhou 310006, Zhejiang, China ∥Zhejiang Entry-Exit Inspection and Quarantine Bureau of P.R.C. International Travel Healthcare Center, Hangzhou 310012, Zhejiang, China S *Supporting Information ABSTRACT: Protein lysine succinylation, an emerging protein post-translational modification widespread among eukaryotic and prokaryotic cells, represents an important regulator of cellular processes. However, the extent and function of lysine succinylation in Mycobacterium tuberculosis, especially extensively drug- resistant strain, remain elusive. Combining protein/peptide prefractionation, immunoaffinity enrichment, and LC−MS/MS analysis, a total of 686 succinylated proteins and 1739 succinylation sites of M. tuberculosis were identified, representing the first global profiling of M. tuberculosis lys