心理应激性内皮损伤动物模型的初步研究.docVIP

精品论文 参考文献 心理应激性内皮损伤动物模型的初步研究 杨晓梅1 庞广保2 沈斌2 韦伍英2 黎木兰2 封廖芸2 李冠虹3 石殿春3 黎静1* （1广西医科大学生理学教研室 530021；2广西医科大学2008级七年制临床医学专业 530021） （3广西医科大学2009级五年制临床医学专业 530021） 【中图分类号】R742【文献标识码】A【文章编号】1672-5085（2012）19-0052-02 【摘要】目的 采用愤怒心理应激建立血管内皮损伤动物模型，并探讨血管内皮损伤的情况。方法 雄性SD大鼠随机分为对照组、模型组，采用愤怒心理应激建立血管内皮损伤模型，放射免疫法检测血浆皮质醇的含量，采用ELISA法检测血浆肾上腺素、血管性血友病因子（vWF），硝酸还原酶法检测血浆NO含量，HE染色及扫描电镜观察血管内皮结构的损伤情况。结果 模型组血浆皮质醇、肾上腺素、vWF水平明显高于对照组（Plt;0.05），血浆NO含量明显低于对照组（Plt;0.05），扫描电镜观察血管内皮细胞明显受损。结论 该模型是较理想的心理应激内皮损伤模型。 【关键词】心理应激 血管内皮细胞 肾上腺素 血管性血友病因子 一氧化氮 Exploration animal model of vascular endothelial injured by psychological stress Yang Xiao-mei1, Pang Guang-bao2, Shen Bing2, Wei Wu-ying2, Li Mu-lan2, Feng Liao-yun2, Shi Dianchun3, Li Guanhong3, Li Jing1 （1 Guangxi Nanning, Department of Physiology, 530021，China, 2 Guang Xi Medical University 7-year program clinical medical speciality of 2008 Grade, Guangxi Nanning, 3 5-year program clinical medical speciality of 2009 Grade.） 【Abstract】Objective To establish animal model of endothelial cell injured by psychological stress of anger. METHODS: To set up the animal model of endothelial cell injured by psychological stress of anger, adult male SD rats were randomly divided into control group and model group. The plasma level of cortisol was detected by Radioimmunoassay, the plasma level of epinephrine and vWF detected by ELISA. The plasma NO levels dectected by nitrate reductase assay. The morphology of thoracic aorta endothelium was observed by HE stain and scanning electron microscopy. RESULTS: The plasma levels of cortisol, epinephrine and vWF in the model group were significantly higher than those of control group(Plt;0.05). The plasma levels of NO was significantly lower than that of the control group (Plt;0.05). And the morphology of the endothelial cell was significantly impaired. CONCLUSION: Psychological stress was an ideal way to establish animal model of endothelial cell injured.