一株产木聚糖酶放线菌的液体发酵条件优化及水解特性-河南农业科学.PDFVIP

※生物工程 食品科学 2012, Vol.33, No.21 177 1,2 1 1,3,* 1,2 1 1 朱运平 ，禇文丹 ，李秀婷 ，滕 超 ，李 娥 ，杨 然 (1.北京工商大学食品学院，北京 100048；2.食品添加剂与配料北京市高等学校工程中心，北京 100048； 3.北京市食品风味化学重点实验室，北京 100048) 摘 要：以木聚糖为唯一碳源制作选择培养基，利用透明圈法筛选高产木聚糖酶菌株，对其中一株产酶较高的 菌株L10608进行液体发酵条件优化并对所产木聚糖酶的水解特性进行研究。结果表明：菌株L10608最佳产酶条 件为以质量浓度25g/L 、80 目的水不溶性玉米芯木聚糖为碳源，10g/L大豆蛋白胨和5g/L酵母浸膏为复合氮源， 初始pH6.0 、培养温度40 ℃、转速200r/min 、表面活性剂吐温-80质量浓度4g/L ，最佳产酶条件下木聚糖酶活力达 1074.8U/mL。以桦木木聚糖、榉木木聚糖和燕麦木聚糖为底物研究菌株L10608所产木聚糖酶的水解特性，结果表 明该木聚糖酶为内切型木聚糖酶，水解主要产物为木二糖和木三糖。表明菌株L10608有望作为功能性低聚木糖的 生产菌株。 放线菌；木聚糖酶；液体发酵；条件优化；低聚木糖 Optimization of Liquid Fermentation Conditions for Xylanase Production by a Actinomyces and Characterization of the Enzyme 1,2 1 1,3,* 1,2 1 1 ZHU Yun-ping ，CHU Wen-dan ，LI Xiu-ting ，TENG Chao ，LI E ，YANG Ran (1. School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing 100048, China； 2. Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients, Beijing 100048, China ； 3. Beijing Key Laboratory of Flavor Chemistry, Beijing 100048, China) Abstract In the current study, high-xylanase-producing strain was screened from different soil samples by transparent circle method, using xylan as the only carbon source in medium. The cultural condition for xylanase production by strain L10608 was optimized and the hydrolysis property of the enzyme was further investigated. The results indicated that the optimum fermentation medium contained a