一株具有拮抗作用的海洋放线菌筛选及其特性-食品与生物技术学报.PDFVIP

第29 卷第3 期 食 品 与生 物 技 术学 报 Vol. 29 No.3 2010 年5 月 Journal of Food Science and Biotechnology May 2010 :1673-1689( 2010)03-0471-05 一株具有拮抗作用的海洋放线菌筛选及其特性 黄惠莉, 肖 易, 殷 果 (华侨大学 化工学院, 福建厦门361021) : 为了探寻对大肠杆菌金黄色葡萄球菌具 拮抗作用效果的海洋微生物新菌种, 作者采用 平板稀释方法, 经初筛与复筛从海洋淤泥中分离得到 一株对大肠杆菌和金黄色葡萄球菌具 较好 拮抗作用效果的海洋微生物菌L 菌株,并对该菌的生理生化特性发酵过程参数的变化及抑菌效 果进行了研究结果表明:海洋微生物菌L 菌经初步判断属放线菌,L 菌适宜的发酵时间为9~ 10 d, 产生的发酵产物对大肠杆菌和金黄色葡萄球菌均 一定的拮抗作用 : 海洋微生物; 次生代谢产物; 抑菌率 : TQ 920. 1 : A Characteristic of Marine Actinomycete Having Antagonistic Effects HUANG Hu-i li, XIAO Yi, YIN Guo ( Chemical Engineering College, Huaqiao University, Xiamen 361021, China) Abstract:The purpose of this manuscript is to screen the marinemicroorganisms and evaluate the antagonistic effects of those isolated microorganism to Escher ich i a col i and the S tap hy lococc us . A isolated marine microorganism L having better antagonism effects was separated from the diluted marine mud. Physiology and biochemical characteristics of the isolated marine microorganism, thecurveof fermentation controlparameters were studied. Antagonism effects of the ferment production were testedwith the Escher ic hi a col i and the S tap hy lococc us . The result shows that marine microorganism L was marine actinomycetes, the suitable fermentation time is 9 to10 days, thesecondary metabolitein fermentation broth havecertainty the antagonism ability to E sche rich ia c ol i and S tap hy lococc us . Key words: marine microorganism, secondary metabolite, ant-i microbial ratio , , 150 , , [ 1- 7] 72 000,