一步法合成二甲醚整体式催化剂的制备及反应性能研究-燃料化学学报.PDFVIP

Rice Science, 2011, 18(4): 250−256 Copyright © 2011, China National Rice Research Institute Published by Elsevier BV. All rights reserved Prokaryotic Expression of Rice Ospgip1 Gene and Bioinformatic Analysis of Encoded Product 1, 2 1 2 1 1 2 CHEN Xi-jun , LIU Xiao-wei , ZUO Si-min , MA Yu-yin , TONG Yun-hui , PAN Xue-biao , XU Jing-you1 1 2 ( Horticulture and Plant Protection College, Yangzhou University, Yangzhou 225009, China; Key Laboratory of Plant Functional Genomics of Ministry of Education / Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Yangzhou University, Yangzhou 225009, China) Abstract: Using the reference sequences of pgip genes in GenBank, a fragment of 930 bp covering the open reading frame (ORF) of rice Ospgip1 (Oryza sativa polygalacturonase-inhibiting protein 1) was amplified. The prokaryotic expression product of the gene inhibited the growth of Rhizoctonia solani, the causal agent of rice sheath blight, and reduced its polygalacturonase activity. Bioinformatic analysis showed that OsPGIP1 is a hydrophobic protein with a molecular weight of 32.8 kDa and an isoelectric point (pI) of 7.26. The protein is mainly located in the cell wall of rice, and its signal peptide cleavage site is located between the 17th and 18th amino acids. There are four cysteines in both the N- and C-termini of the deduced protein, which can form three disulfide bonds (between the 56th and 63rd, the 278th and 298th, and the 300th and 308th amino acids). The protein has a typical leucine-rich repeat (LRR) domain, and its secondary