奈比洛尔联用氯沙坦对原发性高血压大鼠舒血管效应的影响.docVIP

精品论文 参考文献 奈比洛尔联用氯沙坦对原发性高血压大鼠舒血管效应的影响 张书荷 　　（天津公安消防总队医院 300074） 　　【摘要】目的 观察奈比洛尔对原发性高血压大鼠(SHR)血压以及不同动脉对血管紧张素II受体阻滞剂氯沙坦舒血管效应的影响。方法 将16只SHR随机分成奈比洛尔治疗组(奈比洛尔8 mg.kg-1.d-1灌胃，n=8)和SHR组(灌胃等量蒸馏水，n=8)。以同周龄的雄性正常血压Wistar—Kyoto(WKY)大鼠作为对照(n=8)。给药8周后，无创法测定尾动脉收缩压，处死动物后取胸主动脉、颈动脉、肾动脉、股动脉，观察氯沙坦((10-7～3x10-4M)舒血管效应的变化。结果 与SHR组相比，奈比洛尔可明显抑制SHR血压升高[(152士27)mm Hg比(203士36)mm Hg，1 mm Hg=0.133 kPa，Plt;0.05]。SHR组不同动脉(主动脉，肾动脉，股动脉)对氯沙坦的最大舒张效应(Emax)小于WKY组(Plt;0.05)，但奈比洛尔可增加SHR组主动脉，颈动脉，肾动脉，股动脉对氯沙坦的Emax (Plt;0.05)，同时奈比洛尔降低颈动脉对氯沙坦的EC50，而对主动脉、肾动脉和股动脉的EC50则无显著影响。结论 奈比洛尔可增加SHR血管对氯沙坦的舒张效应。 　　【关键词】奈比洛尔 氯沙坦 原发性高血压 大鼠 动脉 　　【中图分类号】R969 【文献标识码】A 【文章编号】1672-5085（2014）27-0189-03 　　【Abstract】Objective To investigate the effects of nebivolol treatment on blood pressure and action of angiotensin II receptor blocker，losartan，on different arteries in rats with primary hypertension．Methods Sixteen rats with primary hypertension were randomly divided into two groups：nebivolol (n=8)and primary hypertensive groups(n=8)．In the nebivolol group，nebivolol was given by intragastric lavage for 8 weeks，the dosage being 8mg.kg-1.d-1．In the primary hypertensive group，the same amount and method of administration of distilled water was applied．Eight male normotensive Wistar Kyoto(WKY)rats with same age were used as controls．After 8 weeks of treatment，systolic blood pressure was measured by tail—cuff method(without trauma)．The rats were sacrificed，and the vascular relax responses to losartan (10-7 ~3x10-4M)were tested on chest aorta，carotid artery，renal artery and femoral artery rings．Results Compared with primary hypertensive group，the systolic blood pressure was gradually decreased after treatment in nebivolol group[(152士27)mm Hg vs．(203土36)mm Hg，1 mm Hg=0.133 kPa, Plt;0.05] The maximum relax response to losartan (Emax)of different arteries in primary hypertensive group was smaller than that in WKY control group. Treatment with nebivolol increased the relaxation of dif