溶磷和pH值对杀真菌素链霉菌合成恩拉霉素的影响.PDFVIP

28 4 河 北 工 业 科 技 Vol. 28, No . 4 2011 7 H ebei Journal of Industrial Science and T echnolog Jul 20 11 : 2011) pH , ( 河北圣雪大成制药有限责任公司, 河北石家庄 051430) : 研究了培养基灭菌后溶 和发酵过程pH 值等对杀真菌素链霉菌( St rep t omy c es f ung ici d ic us ) JZ14056D3 合成恩拉霉素的影响, 通过发酵尾气成分的测量和OUR, DO 等参数的分析, 发 现培养基灭菌后溶 质量浓度控制在065 g/ mL, 控制发酵过程pH 值为7. 0, 192 h 发酵单位最 高可达5 7 10 U/ mL : 恩拉霉素; 发酵; pH 值; 溶 : T Q465. 6 : A Investigation for effects of dissolved phosphate and pH on producing enram cin b St r ep tomy cesf ung ic id icus JZ14056D3 LIU T iejun, LI Jiong ( Hebei Shengxue Dacheng Pharmaceutical Com pan Limited, Shijiazhuang H ebei 05 1430, China) Abstract: This paper investigated the effects of dissolved phosphate concentration and pH in the fermentation medium after sterilization on the product ion of enram cin b Str ep tomy cesf ung ic id ic us JZ14056D3. T hrough the assa ing of offgas and anal sis of OUR and DO, it is found that the ield of enram cin can reach 5 710 U/ mL in 192 h w hen the dissolved phosphate concentrat ion in the medium is 065 g/ mL and the pH is 7. 0. Key words: enram cin; fermentastion; pH values; dissolved phosphate ( enram cin) ( enduraci , din) , ( S tr ep t omy ces f ung icid i , cus ) , , A B( 7 3) 1966 , 1974 , , [ 1] , 1. 0 mmol/ L, 10 mmol/ L , [ 2] , pH , [ 3]