二硫苏糖醇抑制乳球蛋白淀粉样纤维的形成.pdfVIP

Biophysics 生物物理学, 2014, 2, 39-44 Published Online November 2014 in Hans. /journal/biphy /10.12677/biphy.2014.24005 Dithiothreitol Inhibits the Amyloid Fibril Formation of β-Lactoglobulin Baoliang Ma, Liangshu Wei, Liping Lu, Fang Wu Department of Physics, Science of College, Nanjing Agricultural University, Nanjing Email: mabaoliang@ th th th Received: Sep. 9 , 2014; revised: Oct. 4 , 2014; accepted: Oct. 10 , 2014 Copyright © 2014 by authors and Hans Publishers Inc. This work is licensed under the Creative Commons Attribution International License (CC BY). /licenses/by/4.0/ Abstract Amyloid fibrils are associated with Alzheimer’s disease, Parkinson’s disease, and non-neuropathic systemic amyloidosis. The effect of DTT on the amyloid formation of β-lactoglobulin has been ex- amined by thioflavin T binding, Congo red binding and transmission electron microscopy. The re- sults showed that the formation of the amyloid fibril took place when β-lactoglobulin was incu- bated in the presence of 5M urea and at neural pH. However, the formation of amyloid fibrils was inhibited when DTT was added into the solutions and the inhibitory effect is dependent on the concentrations of DTT. These results demonstrate that the disulfide bonds play important roles in the formation of amyloid fibrils. Keywords β-Lactoglobulin, Disulfide Bonds, Amyloid Fibrils, Transmission Electron Microscopy, Dithiothreitol (DTT) 二硫苏糖醇抑制乳球蛋白淀粉样纤维的形成 马保亮，魏良淑，卢礼萍，吴 芳 南京农业大学理学院物理系，南京 Email: mabaoliang@ 收稿日期：2014年9月9 日；修回日期：2014年10月4 日；录用日期：2014年10月10 日 39 二硫苏糖醇抑制乳球蛋白淀粉样纤维的形成 摘 要 淀粉样纤维与老年痴呆症、帕金森病和非神经性组织淀粉样变性病等人类