京尼平交联的丝素蛋白-壳聚糖缓释微球的制备与-南方医科大学学报.pdfVIP

J South Med Univ, 2014, 34(6): 875-879 doi 10.3969/j.issn. 1673-4254.2014.06.24 ·875 · 基础研究 京尼平交联的丝素蛋白京尼平交联的丝素蛋白--壳聚糖缓释微球的制备与表征壳聚糖缓释微球的制备与表征 1 1 1 1 1 1 2 叶漫文 ，曾曙光 ，高文峰 ，容明灯 ，郭泽鸿 ，石 勇 ，干辉勇 1 2 南方医科大学附属广东省口腔医院，广东 广州 510220；东莞市三局医院，广东 东莞 523710 摘要：目的 以京尼平交联制备丝素蛋白-壳聚糖微球，以牛血清白蛋白（BSA）作为模型蛋白，评价丝素蛋白-壳聚糖微球的缓释 能力。方法 以无机乳化交联法，京尼平为交联剂，制备缓释BSA 的丝素蛋白-壳聚糖微球，通过扫描电镜观察微球表面形态并 测量微球的粒径，X-射线衍射及傅里叶红外光谱对微球表征分析，以BCA 法测定微球的包封率，载药率和21 d 的累积缓释率。 结果 以京尼平交联制备的丝素蛋白-壳聚糖微球，微球形态较为规则，表面较光滑，粒径分别为7.84± 0.97 μm ，包封率为 50.16%±4.32% ，载药率为1.25%±0.11% ，21 d 的累积释放率为75.2%±2.53%。结论 以京尼平交联制备的丝素蛋白-壳聚糖微球 载药率高，缓释能力显著。 关键词：丝素蛋白；壳聚糖；京尼平；微球 Preparation and characterization of genipin-crosslinked silk fibroin/chitosan controlled-release microspheres 1 1 1 1 1 1 2 YE Manwen , ZENG Shuguang , GAO Wenfeng , RONG Mingdeng , GUO Zehong , SHI Yong , GAN Huiyong 1Guangdong Provincial Stomatological Hospital Affiliated to Southern Medical University, Guangzhou 510220, China; 2 San Ju Hospital, Dongguan 523710, China Abstract: Objective To investigate the property of genipin-crosslinked silk fibroin(SF)/chitosan(CS) microspheres for slow releasing of bovine serum albumin (BSA). Methods BSA-loaded genipin-crosslinked SF/CS microspheres were prepared by emulsion cross-linking technique. The micropheres were observed for surface morphology and size distribution under scanning electron microscope (SEM), and X-ray diffractometry (XRD) and fourier transform infrared spectroscopy (FTIR) were used to analyze their structural characteristics. BCA method was used for determining the drug entrapment, loading rate and cumulative drug release in 21 days. Results The microspheres were