拉曼光谱法检测灵芝孢子油氧化酸败-食品科学.pdfVIP

200 2015, Vol.36, No.24 食品科学 ※安全检测 拉曼光谱法检测灵芝孢子油氧化酸败 能 静，项延楠，孙培龙* （浙江工业大学海洋学院，浙江杭州 310014 ） 摘  要：采用拉曼光谱法直接监测灵芝孢子油中氧化酸败过程中不饱和双键特征峰的变化规律，研究了特征峰的强 度与孢子油氧化酸败程度的关系。结果表明，特征峰强度随孢子油酸值、过氧化值的增加而下降，特征峰的面积与孢 子油酸值之间存在着良好的线性关系，并进一步测定了灵芝孢子油氧化酸败的动力学参数。建立了用拉曼光谱快速检 测灵芝孢子油氧化酸败度的新方法。对该方法的有效性进行评估，并用该方法筛选出有效的灵芝孢子油抗氧化剂。 关键词：拉曼光谱法；灵芝孢子油；氧化酸败；快速检测 Rapid Detection of Oxidative Rancidity of Ganoderma lucidum Spore Oil by Raman Spectroscopy * NENG Jing, XIANG Yannan, SUN Peilong (Ocean College, Zhejiang University of Technology, Hangzhou 310014, China) Abstract: In this work, Raman spectroscopy was employed to directly monitor the changes in the characteristic peaks of the unsaturated double bonds of Ganoderma lucidum spore oil during oxidative rancidity and the relationship between the peak intensity and oxidative rancidity was discussed. Raman scattering intensity decreased with increasing acid value and peroxide value and a linear correlation between acid value and the area of the characteristic peaks was determined. The kinetic parameters for the oxidative rancidity of Ganoderma lucidum spore oil were also determined. As a result, a new and fast approach for the detection of oxidative rancidity in Ganoderma lucidum spore oil by Raman spectroscopy was established. This method was further validated and used to measure the kinetic parameters of oxidative rancidity and screen antioxidants for Ganoderma lucidum spore oil. Key words: Raman spectroscopy; Ganoderma lucidum spore oil; oxidative rancidity; rapid detection 中图分类号：TS207.3 文献标志码：A 文章编号：1002-6630 （2015 ）24-0200-05 doi:10.7506/spkx1002-6630-201524037