水分亏缺条件下玉米根系pip2－5基因的表达-黄土高原土壤侵蚀与.pdfVIP

植物生理学通讯 第42卷 第3期，2006年6月 4 5 7 水分亏缺条件下玉米根系PIP2-5基因的表达 1,2,3 1,* 1 1 吴安慧 张岁岐 邓西平 山仑 1 2 中国科学院水利部水土保持研究所黄土高原土壤侵蚀与旱地农业国家重点实验室， 陕西杨凌 712100； 中国科学院研 3 究生院，北京100039； 西北农林科技大学植物保护资源与病虫害治理教育部重点实验室，陕西杨凌712100 提要 在PEG-6000模拟水分亏缺条件下，以微管蛋白基因为内参基因，水通道蛋白基因PIP2-5为检测基因，采用半定 量逆转录聚合酶链式反应(RT-PCR)体系检测PIP2-5基因在玉米根系中的表达的结果表明，人工模拟水分亏缺条件下PIP2- 5基因表达量高于正常水分条件下的。这暗示，水分亏缺条件下细胞-细胞途径对根系吸水的贡献可能增大。 关键词 玉米；根系；水通道蛋白；半定量PCR Expression of PIP2-5 in Maize Root Systems under Water Deficit 1,2,3 1, * 1 1 WU An-Hui , ZHANG Sui-Qi , DENG Xi-Ping , SHAN Lun 1State Key Laboratory of Soil Erosion and D ry land Farming on Loess Plateau, Institute of Water and Soil Conservation, Chinese A cademy of Sciences, M inistry of Water R esources, Yangling, Shaanx i 712100, China; 2 Graduate University, Chinese A cademy of Sciences, Beij ing 100039, China; 3Key Laboratory of Plant Protection Resources and Pest Management, Ministry of Education, Northwest Sci-Tech University of Ag riculture and Forestry, Yangling, Shaanxi 712100, China Abstract To detect the expression of PIP2-5 in maize root systems under water deficit, semi-quantitative PCR was used. The reference gene was tubulin. Maize seedlings were planted under the condition of hydroponics culture in growth chamber. Water deficit was simulated by using PEG-6000. The results showed that PIP2-5 was up-regulated under water deficit, which supported the theory that the contribution of cell-to-cell pass-way for root systems uptake maybe increased under water deficit. Key words maize; root systems; water channel protein ; semi-quantitative PCR 植物根系的抗旱性可以从生理、形态、解剖 组成。但这个 三个方面来研究。近年来发现质膜和液泡膜上专 家族基因的表达和调节以及相应的蛋白质功能的信 一性、高效性水分跨膜运输的通道— — 水通道蛋 息依然有限。仅有4个玉米水通道蛋白的运输活 白(aquaporin, AQP)，它介导细胞或细胞器与介质