新西兰白兔脑电生物学特性研究-中国试验动物学报.docVIP

研究报告 新西兰兔脑电的生物学特性 恽时锋，方 天，董 敏，赵志刚，梁 磊，许龙祥，陈 莉，刘 彪， 吴正林，田小芸， 周森妹 （南京军区南京总医院比较医学科， 全国科普教育基地，南京 210002) 【摘要】～1α波及16～β波为基本节律，波幅为30～μV；ECoG则以10～α波及16～β波为基本节律，波幅为200～μV；麻醉能使脑电活动减弱，脑组织缺氧，麻醉后ECoG以δ及θ慢波为主。结论EEG测定方法简单，操作简便，家兔无需麻醉，无创伤，所描记的脑电波形与ECoG 高度一致，可以替代传统的ECoG进行神经系统功能的研究。 【关键词】头皮；脑电图 【中图分类号】R33【文献标识码】A【文章编号】1671-7856（201）-0000-00 Doi:10.3969/j.issn.1671-7856.2014.006.000 Biological characteristics of rabbit electroencephalography (EEG)YUN Shi-feng，FANG Tian, DONG Min, ZHAO Zhi-gang, LIANG Lei, XU Long-xiang, CHEN Li, LIU Biao, WU Zheng-lin, TIAN Xiao-yun, ZHOU Seng-mei （Department of Comparative Medicine, National Science Education Base, Nanjing 210002, China）【Abstract】Objective the extracranial scalp electroencephalography (EEG) and intracranial electrocorticography (ECoG) of closed colony New Zealand white rabbits. Methods To record the extracranial scalp EEG and intracranial ECoG of closed colony New Zealand white rabbits, and to compare and analyze the results of those two scanning methods. Results EEG was characteristic of 9-12 c/s α wave and 16-20 c/s β wave with an amplitude of 30-100 μV as the basic rhythm. ECoG showed 10-12 c/s α wave and 16-20 c/s β wave with an amplitude of 200-300 μV as the basic rhythm. Anesthesia could attenuate the electrocerebral activity, cause brain tissue hypoxia, and induce δ wave and slow θ wave in ECoG. Conclusions EEG method is a simple, non-invasive and convenient operation, and can be made in rabbits without anesthesia. The recorded EEG waveform is highly consistent with that of ECoG, and may be used as an alternative to the traditional ECoG in neurofunctional studies. 【Key words】New Zealand white rabbits; Body temperature; Qualified Rate; Electroencephalography; Electrocorticography 家兔是医学研究中常用的啮齿类实验动物，在神经系统功能的实验研究中，经常需要观察动物机体的脑电活动，而脑电图则是动物机体在清醒安静的状态下，大脑皮层自发的具有持续节律性的电活动，是脑电活动的真实记录。在一般情况下，由于动物与人类的配合程度欠佳，动物脑电图的描记采用开颅术，直接记录ECoG[1-4]，但