中药天年饮对脾衰老延缓作用探究.docVIP

中药天年饮对脾衰老延缓作用探究 　 作者：陈志宏 程丽梅 张宏凯 谢利德 高福禄 【摘要】 目的 观察脾的衰老变化及中药天年饮延缓免疫器官衰老的作用。方法 D半乳糖连续腹腔注射建立亚急性衰老的大鼠模型；待模型成功建立后，用药组大鼠给予天年饮灌胃。分别检测各组大鼠血脂和脾脏指数的变化，观察脾增殖细胞核抗原(PCNA)、p53和p16表达的变化。结果 与正常组大鼠比较，模型组大鼠的血脂和脾p53、p16的表达明显升高，模型组的脾脏指数和脾PCNA的表达明显下降(P＜0.05，P＜0.01)。中药天年饮可明显改善模型大鼠脾的各种变化：与模型组大鼠比较，天年饮用药组大鼠血脂和脾p53、p16的表达明显下降，而脾脏指数和脾PCNA的表达明显升高(P＜0.05，P＜0.01)。结论 天年饮可通过阻止脾脏的萎缩、促进淋巴细胞的增殖能力，提高衰老机体的免疫功能，可能具有延缓衰老的作用。 【关键词】 衰老；脾；天年饮；PCNA；p53；p16 【Abstract】 Objective To observe the senescent changes of spleen and the antiaging effects of traditional Chinese drug Tiannianyin on immune organs.Methods 30 SD rats were randomly divided into 3 groups and each group had 10 rats: normal, aging model and Tiannianyin groups. The subacutely aging model rats were made by injecting Dgal into abdominal cavity continually. After the aging model was successfully established, the rats in Tiannianyin group were administrated Tiannianyin by intragastric administration. The blood lipid and the spleen index of rats in each group were measured, and the expressions of PCNA, p53 and p16 of spleen were observed respectively. Results Compared with normal rats, the spleen index and the expression of PCNA in spleen of aging model rats decreased obviously; while the blood lipid, the expressions of p53 and p16 in spleen of aging model rats increased obviously (P＜0.05, P＜0.01). Tiannianyin could improve the senescent changes in spleen of aging rats significantly: compared with aging model rats, the spleen index and the expression of PCNA in spleen increased remarkably and the blood lipid, the expressions of p53 and p16 in spleen decreased in Tiannianyin group remarkably (P＜0.05, P＜0.01). Conclusions Tiannianyin can improve the immune function of aged by preventing atropy of spleen and facilitating the reproductive activity of lymphocytes, thus has better antiaging effects. 【Key words】 Aging; Spleen; Tiannianyin; PCNA; p53; p16 1988年Meites〔1〕提出，在衰老的过程中，免疫神经内分泌网络起着重要作用。淋巴器官功能降低是免疫功能紊乱的主要原因,也与免疫功能的衰退密切相关〔2〕；因此，研究免疫系统的衰老